LOCUS       sequence-120476-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IL1B
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..36
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctataca agctggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagttggc
       61 atggcagaag tacctgagct cgccagtgaa atgatggctt attacagtgg caatgaggat
      121 gacttgttct ttgaagctga tggccctaaa cagatgaagt gctccttcca ggacctggac
      181 ctctgccctc tggatggcgg catccagcta cgaatctccg accaccacta cagcaagggc
      241 ttcaggcagg ccgcgtcagt tgttgtggcc atggacaagc tgaggaagat gctggttccc
      301 tgcccacaga ccttccagga gaatgacctg agcaccttct ttcccttcat ctttgaagaa
      361 gaacctatct tcttcgacac atgggataac gaggcttatg tgcacgatgc acctgtacga
      421 tcactgaact gcacgctccg ggactcacag caaaaaagct tggtgatgtc tggtccatat
      481 gaactgaaag ctctccacct ccagggacag gatatggagc aacaagtggt gttctccatg
      541 tcctttgtac aaggagaaga aagtaatgac aaaatacctg tggccttggg cctcaaggaa
      601 aagaatctgt acctgtcctg cgtgttgaaa gatgataagc ccactctaca gctggagagt
      661 gtagatccca aaaattaccc aaagaagaag atggaaaagc gatttgtctt caacaagata
      721 gaaatc
//
