LOCUS       sequence-120491-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: RXFP4-Tango
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(365..376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gctctctggc taactagaga acccactgcc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc
      121 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg cgccaccatg
      181 aagacgatca tcgccctgag ctacatcttc tgcctggtat tcgccgacta caaggacgat
      241 gatgacgcca gcatcgatat gccaactctt aacacatccg cctccccccc cacctttttc
      301 tgggcaaacg ctagcggcgg ttccgtgctc tcagcggacg atgccccaat gcccgtgaag
      361 tttctcgccc ttcgattgat ggtggctttg gcatatggct tggttggcgc tattggcctc
      421 ctgggcaacc tggccgtgct gtgggtgctg tcaaactgtg cccgacgagc gccagggcct
      481 cctagtgaca cattcgtatt taatctcgcc ctggcggacc tgggcctggc gctgaccttg
      541 cccttctggg cagctgaaag tgcccttgat ttccattggc cattcggagg cgctctgtgc
      601 aaaatggtgc tgactgccac tgtgctgaac gtttacgcat ctatattcct gatcaccgcc
      661 ctgtccgtgg ccagatattg ggtggtcgcg atggccgcag gtcccggaac tcacctgtcc
      721 cttttctggg cccggatcgc cactctcgca gtgtgggctg cggctgccct cgttacagta
      781 ccaacagctg tgttcggtgt ggagggagaa gtttgtggtg tgagactgtg tctcctgagg
      841 ttcccctcaa gatattggct gggagcttac caactgcaaa gagtggtgct cgcttttatg
      901 gttccacttg gagtgatcac aacctcctac ctgctgttgt tggcatttct gcaacgacgc
      961 caga
//
