LOCUS       sequence-120511-        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a GlmS/M-domain 
            3'-tag for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Batey RT, Kieft JS
  TITLE     Improved native affinity purification of RNA.
  JOURNAL   RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.
  PUBMED    17548432
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2007-08"; volume: "13(8)"; pages: "1384-9. Epub 2007 Jun 4"
COMMENT     Sequence Label: pRAV24
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      342..389
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(408..424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(408..424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(421..443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    432..448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(456..462,463..480,481..486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    501..522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(639..656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(890..909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aattctaata cgactcacta tagggtcgct caaacattaa gtggtgttgt gcgaaaagaa
       61 tctcgaagac aagccggcat agcgcccgaa ctaccggtac cggtagttga cgaggatgga
      121 ggttatcgaa ttttcggcgg atgcctcccg gctgagtgtg cagatcacag ccgtaaggat
      181 ttcttcaaac caagggggtg actccttgaa caaagagaaa tcacatgatc ttccaaaaat
      241 ctagactgtg catcgggtca ggactgaaag gtagcagccc tgggcagttt tttcctgtgc
      301 atcgggtcag gaccttcggg tagcagccct gggcaggatc cctagcataa ccccttgggg
      361 cctctaaacg ggtcttgagg ggttttttga agcttggcgt aatcatggtc atagctgttt
      421 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      481 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      541 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      601 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga acccccgttc
     1081 agccgacgct gcgccttatc cggtaactat cgtcttg
//
