LOCUS       sequence-120513-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector MBP/hexahistidine tagged variant of the MS2 coat 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Batey RT, Kieft JS
  TITLE     Improved native affinity purification of RNA.
  JOURNAL   RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.
  PUBMED    17548432
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2007-08"; volume: "13(8)"; pages: "1384-9. Epub 2007 Jun 4"
COMMENT     Sequence Label: pHMM
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(424..435)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(469..492)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      600..609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt
       61 gcggccgcaa gcttagtaga tgccggagtt tgctgcgatt gctgagggaa tcgggtttcc
      121 atcttttagg agaccttgca ttgccttaac aataagctcg cagtcggaat tcgtggcgaa
      181 aattggaatg gttagttcca tatttaagta cgaacgccag gcgcctttag gcacctcgac
      241 tttgatggtg tatttgcgat tctgcgcaga gctctgacga acgctacagg ttactttgta
      301 agcctgtgaa cgcgagttag agctgatcca ttcagcgatc ccgttagcga agttgcttgg
      361 ggcgacagtc acgtcgccag ttccgccatt gtcgacgaga acgaactgag taaagttaga
      421 agccctaccc tcgatggatc cccggccggg taccgagctc gaattagtct gcgcgtcttt
      481 cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag tacgcacggc
      541 ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct gggcgttttc
      601 catggtggcg gcaatacgtg gatctttcgc caactcttcc tcgtaagact tcagcgctac
      661 ggcacccagc ggtttgtctt tattaaccgc ttccagacct tcatcagtca gcagatagtt
      721 ttcgaggaac tctttcgcca gctctttgtt cggactggcg gcgttaatac ctgcgctcag
      781 cacgccaacg aacggtttgg atggttgacc cttgaaggtc ggcagtaccg ttacaccata
      841 attcactttg ctggtgtcga tgttggacca tgcccacggg ccgttgatgg tcatcgctgt
      901 ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt
      961 gtttttaatc aggtcaacca ggaaggtcag acccgctttc gcgccagcgt tatccacgcc
     1021 cacgtcttta atgtcgtact tgccgttttc a
//
