LOCUS       sequence-120520-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Complementation in Cryptococcus neoformans. Targets DNA constructs 
            to a Safe Haven Site, a small gene-free region. Contains 
            nourseothricin resistance marker (NAT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Arras SD, Chitty JL, Blake KL, Schulz BL, Fraser JA
  TITLE     A genomic safe haven for mutant complementation in Cryptococcus 
            neoformans.
  JOURNAL   PLoS One. 2015 Apr 9;10(4):e0122916. doi: 
            10.1371/journal.pone.0122916. eCollection 2015.
  PUBMED    25856300
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-9"; volume: "10(4):e0122916. doi"; pages: " 
            10.1371/journal.pone.0122916. eCollection 2015"
COMMENT     Sequence Label: pSDMA25
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..295)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      301 ttccccgaaa agtgccacct gacgcgccct gtagcggcaa gatctgccca tagacttcaa
      361 atcatggaat tggacgttta tcttcttgct ttggatatcc accctatcgt acaatcttgg
      421 cactttggat catacgaggc actttgcgga ctagggaatg gttatcatca atttcttgga
      481 cctctccatg acacctggca tcgtcgagct accacagccc cctgaattct taaaggcgaa
      541 ccccccttcc taggaacctt gagcgctgga ctggatggca ctctgagacc gcctatgctt
      601 aaggacgcga gcttaccctt ccattgatgg aagtcgatca tttatgataa tagaacacgg
      661 cggaacttga ctttacaggc caagggaagc aacaggagaa gaagctaagg ttataacagg
      721 ctcagcatat ttgtatatac ggtgccattc aacgctgaac actttgtact cacggtctgc
      781 ccgttgaata acaccatctg ctgatgatca tcccaatttg tgagtcttat ttgccattga
      841 tcctggtgta taaccgatac tgaagccctt taggtctgat cagttataga cgtggtatat
      901 tcattcttcc tagattagct atgccgaaag agatggatac cttgatgtga ctggaatgcc
      961 atgaagcagg agattgtagc agctcaaatt ggagaaggat gttgcaaccg tc
//
