LOCUS       sequence-120523-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Complementation in Cryptococcus neoformans. Targets DNA constructs 
            to a Safe Haven Site, a small gene-free region. Contains HYG 
            resistance marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Arras SD, Chitty JL, Blake KL, Schulz BL, Fraser JA
  TITLE     A genomic safe haven for mutant complementation in Cryptococcus 
            neoformans.
  JOURNAL   PLoS One. 2015 Apr 9;10(4):e0122916. doi: 
            10.1371/journal.pone.0122916. eCollection 2015.
  PUBMED    25856300
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-9"; volume: "10(4):e0122916. doi"; pages: " 
            10.1371/journal.pone.0122916. eCollection 2015"
COMMENT     Sequence Label: pSDMA58
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     44..64
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        46..64
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    77..184
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     101..117
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     101..117
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(151..167)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(152..168)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(192..211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(193..211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(221..238)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(221..237)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(230..252)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
       61 aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc
      121 cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
      181 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      241 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      301 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      361 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      421 ttgttaaatc agctcatttt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      481 caaaagaata gaccgagata gggttgagtc catggctgcg aggatgtgag ctggagagcg
      541 gcgcacggga aggggaggac tcacataagc atgcaggatt cgagtggcat ggtgtgcact
      601 gagtgtatgg ttgtcggagg agaggatgat ggtaacaaca atagcagcaa cgtcactcga
      661 cgcgcgtccg gtgtgccaca cggggtaacg ccgagtcgcc gtcagggtcg ccgagaccac
      721 tctcacagcg tcaccgttgg caccagctca gcttacagct tctatcctcc gccagcatcc
      781 acatacatcc cctataccgc atcccccacc cactgcccaa ggtgagtcat cttcccgccc
      841 ccttcccttg cccgccactc agtcctccat cctccactaa tccaccttat cgcacccacc
      901 gcctatcgca catccgagca caatgctggg cctgccaggg gctgctagat ggtgctctcc
      961 ccacgctgat ctgcatgccg gccattggat catgggtgct aggtgctggg tgctggatgt
     1021 tggatgctgg atgctgggtg cacgcttggt catttccttc caggattgac ggtcgccgag
     1081 aggacgacgt ggcgttc
//
