LOCUS       sequence-120531-         415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 EBNA1BP2
FEATURES             Location/Qualifiers
     source          1..415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..36
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gagctataca agctggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagttagc
       61 atggacactc ccccgctctc ggattcggag tcggaatccg atgaatccct tgtcacagac
      121 agagagttgc aggatgcgtt ttcccgaggg cttctgaagc caggcctcaa tgtcgtgcta
      181 gaggggccga agaaggccgt gaacgacgtg aatggcctga agcaatgttt ggcagaattc
      241 aagcgggatc tggaatgggt tgaaaggctc gatgtgacac tgggtccggt accggagatc
      301 ggtggatctg aggcgccagc acctcagaac aaggaccaga aagctgttga tccagaagac
      361 gacttccagc gagagatgag tttctatcgc caagcccagg ccgcagtgct tgcag
//
