LOCUS       sequence-120534-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TCF3
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(585..609)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(610..633)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(676..697)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg caacatgtgc ccggcgggac cttccggacc ttcttgggct gcgtgtctag
      241 gctgccgtct gccgctctcc gccgggagct gccggagtag gaggggtagt actgggaggt
      301 ccccttcatg cccgaagggg acaggggccc ggggctgttg agggccagct cgcctgacag
      361 gaagccagcc tgagtcaggc cgcccacgcc tgcgtctctc ccgaaggagg cataggcgcc
      421 ccgctcaccg ctcttgcctg aacctccgaa ctgcgccccg gccagggagg cgggccggcc
      481 cttcccgttg gtgacaggca gcgggaacat catgctgaag tccaggaggt cactgagctc
      541 cttgtctgtg cccacaggcg ccatcctctg cggctggttc atggtgccaa cttttttgta
      601 caaacttgtc ggaccctgga agagcacctc cagcttgtat agctcgtcca tgccgagagt
      661 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      721 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      781 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      841 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      901 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt ctt
//
