LOCUS       sequence-120537-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 RRN3
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg caagagggga ctgggttgca tgtacaacac gggtggggag cccacactac
      241 ttgaaggact tcggaaatgc gtgtcaaagg agcttggtgt gatcccaatc acggtatcat
      301 tctggggcac ttcgcctttc agaaagtcat catcttcatc ttccactatg tcctttttca
      361 tgggtttctt gaactcctgt agctcttcag cactcatgtc ttcccacacc tgataaatag
      421 gatcaatgaa tttctttgac ctcttcagca cacagggatc aaaggggaag aaggtgtcca
      481 gcgggtttgt gcagatctgc actgagtctc ctccagcggt actcctaatg actggcagca
      541 tctggcgatt gttcctctca atgatggtgt agcagaagac gagctggtac ttatttgtga
      601 ttgcagcaaa aaagttaacc actgagggca ggcaaatctt caggggattt agctggctca
      661 tcactatccg ctcaaaattc agactctgaa gatactgcaa accttctttc aggtttccgc
      721 tcaaaagctg cttgtgtcta aaaacaaagg tgtagaacac agcttggcag gctgagtaaa
      781 atggtccatg gagagcaaca tcgcagaatg cctttgttcc cgaatcctgg ttattaaggt
      841 atatgtgcag ccagttaacc aaaagatcta ggcatgattt tacagtaata agaggaataa
      901 atttagctct tgccaaaaag cttccaatat aatttccagc agcctgcctg atgatggcag
//
