LOCUS       sequence-120538-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 RRN3
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..44)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(188..207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(189..207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        239..343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             528..906
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(562..581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctctcgccc tgcttgtgcc gtggtgatgg tggtgatggt ggtgaccaga ggtgcccatg
       61 gttttcactt acgtgtttaa tgtctttaca gactttatac attactgtca tggttaaata
      121 aaataaaata aaataaaata aaataaaata aaataaaata aaataaaata aaataaactt
      181 aagatgtccc tatagtgagt cgtattagac gtcaggtggc acttttcggg gaaatgtgcg
      241 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      301 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      361 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      421 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      481 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      541 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      601 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      661 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      721 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      781 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      841 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      901 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      961 agactggatg ggaggcggat aaagttgcag
//
