LOCUS       sequence-120539-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 STAT1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtacacttca gacacagaaa tcaactcagt cttgatatat ccagttcctt
      241 tagggccatc aagttccatt ggctctggtg cttcctttgg cctggagtaa tactttccaa
      301 aggcatggtc tttgtcaata tttggataca gatacttcag gggattctca ggaatattct
      361 cagcagccat gactttgtaa ttgcgaatga tgtcagggaa agtaacagca gaaagttctt
      421 tcttcgtgta gggttcaacc gcatggaagt caggttcgcc tccgttctgg gaccgctcca
      481 cccatgtgaa tgtgatggcc ccttcccggg agctctcact gaaccgcagc aggaaggtcc
      541 ccggctgctg gtccttcaac agggcacgct ctcgctcctt gctgatgaag cccatgatgc
      601 acccatcatt ccagagaggg agcaggtgtt ttttaatgag ttctaggatg ctttcaatcc
      661 aaagccagaa gggaaaattt ttatcattta tattttcctt acaaaacctc gtccacggaa
      721 tgagaccatc ggggctggcg ttaggaccaa gaagcttctc tcccaacatg ttcagctggt
      781 ccacattgag acctcttttg gtgacagaag aaaactgcca actcagcact tctgaaagct
      841 gagcccatcg tgcacatggt ggagtcagga
//
