LOCUS       sequence-120542-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TCEB3B
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg caatcgtcgg gagaatcttc ccttgtagtc tcgaattgcc ttggccatca
      241 gcggggccac tttcttggca gcctgtttcc gggttttggc cgcgggcgcc gcgtgctcat
      301 tgctgctgct caggcagggg ttggcccgct tctcagggag ccagcgaagg atgctgctgc
      361 tgctgtctct gccaccgccg ctgctgctct ggctggaggg agtgtggctg cttcccgcgg
      421 gcttggaggc tggcttgatc tccccatttt cggggtcagc gtctcctgca gacttctctt
      481 gcctccttga agtatcataa ggcgtcttgg ccacagattt gaaacagatc atctttgcct
      541 ctctgccgtt ggggttgttt ccacgtgcag atcggatatt cgttgtcatt actctcagcc
      601 gctgctctgg ggcgtccgga agccgcaggt actgctccct ccaagttttg ttttcctgtg
      661 gcttttcttc cttgaagtcc tggaaacaat gattcctccg taattcgtct gtctctctaa
      721 cgagtgcgtg attgtctttc tttctgcgat acagctgatc gggcctccac ccttccagaa
      781 caggttcaag aacccagtag gggacctctc ccacgtcgct gagggcgtcc ggattgtttc
      841 taagcacctg ggcacactgc tggcgcagcg tcggcacctg gagctggcag gcaggcctgg
      901 agcccgagta caccggcatc tt
//
