LOCUS       sequence-120550-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an Lkb1-targeting gRNA and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Chiou SH, Winters IP, Wang J, Naranjo S, Dudgeon C, Tamburini FB, 
            Brady JJ, Yang D, Gruner BM, Chuang CH, Caswell DR, Zeng H, Chu P, 
            Kim GE, Carpizo DR, Kim SK, Winslow MM
  TITLE     Pancreatic cancer modeling using retrograde viral vector delivery 
            and in vivo CRISPR/Cas9-mediated somatic genome editing.
  JOURNAL   Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. 
            Epub 2015 Jul 15.
  PUBMED    26178787
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.264861.115"; journalName: "Genes Dev"; date: 
            "2015-07-15- 15"; volume: "29"; issue: "14"; pages: "1576-85"
COMMENT     Sequence Label: Lenti-sgLkb1/Cre
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(51..126)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(147..395)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(205..224)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(375..395)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    477..594
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagaatgcgg ccgccatgtt aattaagcca taggcgcgcc aaacaaaaaa gcaccgactc
       61 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tttctagctc
      121 taaaacttgt gactgcggcc caccacggtg tttggtcctt tccacaagat atataaagcc
      181 aagaaatcga aatactttca agttacggta agcatatgat agtccatttt aaaacataat
      241 tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat
      301 atctttgtgt ttacagtcaa attaattcca attatctctc taacagcctt gtatcgtata
      361 tgcaaatatg aaggaatcat gggaaatagg ccctcacagt ttaaacagtt cctgcagggc
      421 tagagcgggc ccggtactaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
      481 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
      541 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
      601 atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg
      661 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
      721 tcccc
//
