LOCUS       sequence-120551-         477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a non-targeting gRNA and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Chiou SH, Winters IP, Wang J, Naranjo S, Dudgeon C, Tamburini FB, 
            Brady JJ, Yang D, Gruner BM, Chuang CH, Caswell DR, Zeng H, Chu P, 
            Kim GE, Carpizo DR, Kim SK, Winslow MM
  TITLE     Pancreatic cancer modeling using retrograde viral vector delivery 
            and in vivo CRISPR/Cas9-mediated somatic genome editing.
  JOURNAL   Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. 
            Epub 2015 Jul 15.
  PUBMED    26178787
REFERENCE   2  (bases 1 to 477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.264861.115"; journalName: "Genes Dev"; date: 
            "2015-07-15- 15"; volume: "29"; issue: "14"; pages: "1576-85"
COMMENT     Sequence Label: Lenti-sgNT/Cre
FEATURES             Location/Qualifiers
     source          1..477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(48..123)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(144..392)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(202..221)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(372..392)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aatgcggccg ccatgttaat taagccatag gcgcgccaaa caaaaaagca ccgactcggt
       61 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      121 aaccgcggag ccgaatacct cgcggtgttt ggtcctttcc acaagatata taaagccaag
      181 aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt
      241 aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg tactaatatc
      301 tttgtgttta cagtcaaatt aattccaatt atctctctaa cagccttgta tcgtatatgc
      361 aaatatgaag gaatcatggg aaataggccc tcacagttta aacagttcct gcagggctag
      421 agcgggcccg gtactaacca aactggatct ctgctgtccc tgtaataaac ccgaaaa
//
