LOCUS       sequence-120553-       10512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nuclease competent ST1-Cas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: M-ST1-VPR
FEATURES             Location/Qualifiers
     source          1..10512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             748..4110
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MSDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLV
                     RRTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEE
                     LFIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYG
                     QLRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGK
                     RKYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLL
                     NDLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRID
                     KSGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADG
                     SFSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKT
                     TSSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNED
                     DEKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERC
                     LYTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALD
                     SMDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTLYASRVV
                     LNALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNL
                     WKKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSY
                     QVDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSK
                     FLMYRHDPQTFEKVIEPILENYPNKQINDKGKEVPCNPFLKYKEEHGYIRKYSKKGNGP
                     EIKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQ
                     FDKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRT
                     MPKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQ
                     HIIKNEGDKPKLDF"
     CDS             4123..4143
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4168..4317
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4342..4362
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4792..5148
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5167..5736
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    complement(5741..5765)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5898..5917)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5942..5990
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6192..6620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6279..6298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6489..6510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6629..6649)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6634..6963
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6814..6949
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6876..6895
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7030..7824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7084..7103)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7694..7713
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8000..8121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8037..8056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8091..8110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8170..8186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8170..8186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8183..8205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8194..8210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8218..8224,8225..8242,8243..8248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8263..8284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8401..8418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8572..9160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8652..8671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9331..10122,10123..10191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9954..9973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10192..10296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10371..10390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagg atcgaaccct tgccaccatg agcgacctgg tgctgggcct ggacatcggc
      781 atcggcagcg tgggcgtggg catcctgaac aaggtgaccg gcgagatcat ccacaagaac
      841 agtcgcatct tccctgctgc tcaggctgag aacaacctgg tgcgccgcac caaccgccag
      901 ggtcgccggc ttgctcgccg caagaagcac cggcgcgtgc gcctgaaccg cctgttcgag
      961 gagagcggcc tgatcaccga cttcaccaag atcagcatca acctgaaccc ctaccagctg
     1021 cgcgtgaagg gcctgaccga cgagctgagc aacgaggagc tgttcatcgc cctgaagaac
     1081 atggtgaagc accgcggcat cagctacctg gacgacgcca gcgacgacgg caacagcagc
     1141 gtgggcgact acgcccagat cgtgaaggag aacagcaagc agctggagac caagaccccc
     1201 ggccagatcc agctggagcg ctaccagacc tacggccagc tgcgcggcga cttcaccgtg
     1261 gagaaggacg gcaagaagca ccgcctgatc aacgtgttcc ccaccagcgc ctaccgcagc
     1321 gaggccctgc gcatcctgca gacccagcag gagttcaacc cccagatcac cgacgagttc
     1381 atcaaccgct acctggagat cctgaccggc aagcgcaagt actaccacgg ccccggcaac
     1441 gagaagagcc gcaccgacta cggccgctac cgcaccagcg gcgagaccct ggacaacatc
     1501 ttcggcatcc tgatcggcaa gtgcaccttc taccccgacg agttccgcgc cgccaaggcc
     1561 agctacaccg cccaggagtt caacctgctg aacgacctga acaacctgac cgtgcccacc
     1621 gagaccaaga agctgagcaa ggagcagaag aaccagatca tcaactacgt gaagaacgag
     1681 aaggccatgg gccccgccaa gctgttcaag tacatcgcca agctgctgag ctgcgacgtg
     1741 gccgacatca agggctaccg catcgacaag agcggcaagg ccgagatcca caccttcgag
     1801 gcctaccgca agatgaagac cctggagacc ctggacatcg agcagatgga ccgcgagacc
     1861 ctggacaagc tggcctacgt gctgaccctg aacaccgagc gcgagggcat ccaggaggcc
     1921 ctggagcacg agttcgccga cggcagcttc agccagaagc aggtggacga gctggtgcag
     1981 ttccgcaagg ccaacagcag catcttcggc aagggctggc acaacttcag cgtgaagctg
     2041 atgatggagc tgatccccga gctgtacgag accagcgagg agcagatgac catcctgacc
     2101 cgcctgggca agcagaagac caccagcagc agcaacaaga ccaagtacat cgacgagaag
     2161 ctgctgaccg aggagatcta caaccccgtg gtggccaaga gcgtgcgcca ggccatcaag
     2221 atcgtgaacg ccgccatcaa ggagtacggc gacttcgaca acatcgtgat cgagatggcc
     2281 cgcgagacca acgaggacga cgagaagaag gccatccaga agatccagaa ggccaacaag
     2341 gacgagaagg acgccgccat gctgaaggcc gccaaccagt acaacggcaa ggccgagctg
     2401 ccccacagcg tgttccacgg ccacaagcag ctggccacca agatccgcct gtggcaccag
     2461 cagggcgagc gctgcctgta caccggcaag accatcagca tccacgacct gatcaacaac
     2521 agcaaccagt tcgaggtgga ccacatcctg cccctgagca tcaccttcga cgacagcctg
     2581 gccaacaagg tgctggtgta cgccaccgcc aaccaggaga agggccagcg caccccctac
     2641 caggccctgg acagcatgga cgacgcctgg agcttccgcg agctgaaggc cttcgtgcgc
     2701 gagagcaaga ccctgagcaa caagaagaag gagtacctgc tgaccgagga ggacatcagc
     2761 aagttcgacg tgcgcaagaa gttcatcgag cgcaacctgg tggacaccct ctacgccagc
     2821 cgcgtggtgc tgaacgccct gcaggagcac ttccgcgccc acaagatcga caccaaggtg
     2881 agcgtggtgc gcggccagtt caccagccag ctgcgccgcc actggggcat cgagaagacc
     2941 cgcgacacct accaccacca cgccgtggac gccctgatca ttgcggcttc tagccagctg
     3001 aacctgtgga agaagcagaa gaacaccctg gtgagctaca gcgaggacca gctgctggac
     3061 atcgagaccg gcgagctgat cagcgacgac gagtacaagg agagcgtgtt caaggccccc
     3121 taccagcact tcgtggacac cctgaagagc aaggagttcg aggacagcat cctgttcagc
     3181 taccaggtgg acagcaagtt caaccgcaag atcagcgacg ccaccatcta cgccacccgc
     3241 caggccaagg tgggcaagga caaggccgac gagacctacg tgctgggcaa gatcaaggac
     3301 atctacaccc aggacggcta cgacgccttc atgaagatct acaagaagga caagagcaag
     3361 ttcctgatgt accgccacga cccccagacc ttcgagaagg tgatcgagcc catcctggag
     3421 aactacccca acaagcagat caacgataaa ggcaaggagg tgccctgcaa ccccttcctg
     3481 aagtacaagg aggagcacgg ctacatccgc aagtacagca agaagggcaa cggccccgag
     3541 atcaagagcc tgaagtacta cgacagcaag ctgggcaacc acatcgacat cacccccaag
     3601 gacagcaaca acaaggtggt gctgcagagc gtgagcccct ggcgcgccga cgtgtacttc
     3661 aacaagacca ccggcaagta cgagatcctg ggcctgaagt acgccgacct gcagtttgat
     3721 aagggcaccg gcacctacaa gatcagccag gagaagtaca acgacatcaa gaagaaggag
     3781 ggcgtggaca gcgacagcga gttcaagttc accctgtaca agaacgacct tctgctggtg
     3841 aaggacaccg agaccaagga gcaacagctg ttccgcttcc tgagccgcac catgcccaag
     3901 cagaagcact acgtggagct gaagccctac gacaagcaga agttcgaggg cggcgaggcc
     3961 ctgatcaagg tgctgggcaa cgtggccaac agcggccagt gcaagaaggg cctgggcaag
     4021 agcaacatca gcatctacaa ggtgcgcacc gacgtgctgg gcaaccagca catcatcaag
     4081 aacgagggcg acaagcccaa gttggacttc agcagggctg accccaagaa gaagaggaag
     4141 gtggaggcca gcggttccgg acgggctgac gcattggacg attttgatct ggatatgctg
     4201 ggaagtgacg ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac
     4261 tttgacctcg acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt
     4321 aactctagaa gttccggatc tccgaaaaag aaacgcaaag ttggtagcca gtacctgccc
     4381 gacaccgacg accggcaccg gatcgaggaa aagcggaagc ggacctacga gacattcaag
     4441 agcatcatga agaagtcccc cttcagcggc cccaccgacc ctagacctcc acctagaaga
     4501 atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac ctgcccccca gccttacccc
     4561 ttcaccagca gcctgagcac catcaactac gacgagttcc ctaccatggt gttccccagc
     4621 ggccagatct ctcaggcctc tgctctggct ccagcccctc ctcaggtgct gcctcaggct
     4681 cctgctcctg caccagctcc agccatggtg tctgcactgg ctcaggcacc agcacccgtg
     4741 cctgtgctgg ctcctggacc tccacaggct gtggctccac cagcccctaa acctacacag
     4801 gccggcgagg gcacactgtc tgaagctctg ctgcagctgc agttcgacga cgaggatctg
     4861 ggagccctgc tgggaaacag caccgatcct gccgtgttca ccgacctggc cagcgtggac
     4921 aacagcgagt tccagcagct gctgaaccag ggcatccctg tggcccctca caccaccgag
     4981 cccatgctga tggaataccc cgaggccatc acccggctcg tgacaggcgc tcagaggcct
     5041 cctgatccag ctcctgcccc tctgggagca ccaggcctgc ctaatggact gctgtctggc
     5101 gacgaggact tcagctctat cgccgatatg gatttctcag ccttgctggg ctctggcagc
     5161 ggcagccggg attccaggga agggatgttt ttgccgaagc ctgaggccgg ctccgctatt
     5221 agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac gaatccggcc atttcatcct
     5281 ccaggaagtc catgggccaa ccgcccactc cccgccagcc tcgcaccaac accaaccggt
     5341 ccagtacatg agccagtcgg gtcactgacc ccggcaccag tccctcagcc actggatcca
     5401 gcgcccgcag tgactcccga ggccagtcac ctgttggagg atcccgatga agagacgagc
     5461 caggctgtca aagcccttcg ggagatggcc gatactgtga ttccccagaa ggaagaggct
     5521 gcaatctgtg gccaaatgga cctttcccat ccgcccccaa ggggccatct ggatgagctg
     5581 acaaccacac ttgagtccat gaccgaggat ctgaacctgg actcacccct gaccccggaa
     5641 ttgaacgaga ttctggatac cttcctgaac gacgagtgcc tcttgcatgc catgcatatc
     5701 agcacaggac tgtccatctt cgacacatct ctgttttgaa acccagcttt cttgtacaaa
     5761 gtggttgata tcatctagag cggccgcagg tacctgaata actaaggccg cttcccttta
     5821 gtgagggtta atgcttcgag cagacaaagg gttcgatccc taccggttag taatgagttt
     5881 aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     5941 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg
     6001 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     6061 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     6121 cgcagccaac gtcggggcgg cagaccctgc catagcagat ctgcgcagct ggggctctag
     6181 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6241 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6301 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
     6361 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6421 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6481 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6541 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
     6601 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     6661 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     6721 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6781 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6841 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6901 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6961 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     7021 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     7081 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     7141 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     7201 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     7261 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     7321 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     7381 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     7441 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     7501 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     7561 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     7621 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     7681 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     7741 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     7801 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     7861 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7921 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7981 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     8041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8101 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     8161 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8221 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8281 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8341 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8401 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8461 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8521 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8581 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8641 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8701 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8761 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8821 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8881 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8941 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9001 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9061 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10321 tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc
    10381 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    10441 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    10501 aatctgctta gg
//
