LOCUS       sequence-120561-        9862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4-QF-SL2-mcherry-3'UTR-unc-54.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW08
FEATURES             Location/Qualifiers
     source          1..9862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(6080..6113)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7381..7403)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7503..7522
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7604..7622)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7690..7794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7795..7863,7864..8655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8013..8032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8826..9414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9315..9334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9568..9585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9702..9723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9738..9743,9744..9761,9762..9768)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9776..9792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9781..9803
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgca gctctgagac catgttgatg ggaaattata
       61 tttgaaaact tttgaataaa gttgtataat tgtttttatt ctttaaccta tttatgaatg
      121 ctgtcccgga actgggatat aatttctaac ttgacacaat tattccacct ctgtcttcaa
      181 ggcgacctac acctacctcg cgtctaggtc caacagaaac actgttgaat gaaatcctcg
      241 aattgcaaca atataggatg ctcaggagac tataattcaa aaaacccaac ccaaattagc
      301 ataagcttcc ccaaattgga acagtgaaat actcaatcaa tcactctggc ggttttctcc
      361 acaaagaaca cgaaattgga aaaaggaaaa gattgatttg tttgaaggcg tgaggaaatc
      421 gaaaaaaaat tgtttcaaaa caaatgaaat attttaacga ataaacccag gaatgccact
      481 ttttggtgcc cgcctacaaa aaagtcaact gttcttaaac tcctacatag ttgccctctc
      541 taacgaatgt tgcttttttt aaattttcat tgaatttgat tatcgtctgt atgcgcctac
      601 tctatcagtg gaaaacatgc ccatttccca caaatttact cgtttggagc tcaaaatgtt
      661 aaatggggca tcagggaatt ggcatctcac agaaagtggc ggatgagtta tatgtaataa
      721 taatggtggc tgaagtggaa accgcaaaaa aaggatgtgt atttgagatt ttgcaacgga
      781 atttgaatca gatacacaaa atgagagaaa tttatagatt ctttaaaatc cacctgaagt
      841 cggcaaattt ttgaaaggtc tgttcgttca gtttttcata agttttatga attttgattt
      901 actactgaag ttcgagaaaa gccctttaac cataccaata agaaattatg ttaactttac
      961 aagaatatta acaaaaaatt attaaaaaac tgtgttcccc tggcattatg atatttccac
     1021 cgtcagttct ctattaaaac ttcaactcta cacagtttca actctcaaat gagctgtcca
     1081 tcttttgcct tcaggaaatt atgctaatct gctgatttcg cgcactttca ccacactatt
     1141 tccaaataga aggaggggtg gtgcaacaca cgagacactg caaaaaggtt cagtgaagat
     1201 ttatctactt gtgagatgag agcaggaggg gtgggagaag tgtgatgatt gacgggatga
     1261 caagaacagt gatgtggtgt cgaaatggaa gatgcgctaa tttgtggagt tttatgaaat
     1321 ttgaataatt acatttataa catctactag ttaggcgtct gtgtaggtat tgcctgcctg
     1381 cctacaaggc atatgtacga ggcaggttaa acctcctaga aagctacggt actttcaaac
     1441 cacataacat ttatactggt gtcttttcgc gtcttcttct tgaacatgta gaccctcaaa
     1501 aagctaagtt ttacactgtg tagtttgctt tgaaatctac aaaaattgga ggcacgcctt
     1561 ctggactttg ccgacacgcc tactttctac ctaccaactg cctacttcca cagtctagcg
     1621 tgaactccaa taccatttta aaaattgttt tatctcaaat tttcaatttt taaatccaat
     1681 ttcccagttc taaaaaaaaa attttttttt taatttaaaa actctcatcc tttttaatgc
     1741 tcgttcccat tcggcaagac gtgtaccaaa cacaaaacaa attgactggt gatccatctc
     1801 gaatttctcg tttcaaatta gtcgttgcta tatatattgt cttatgtgtg gaaaacgaat
     1861 aagaaaaatt agagaaagat gagagagcag gggcagaatg aaaatttgca tggaaattga
     1921 agaagatcac tgctttttcc gatgaatgat gactaaatag aatgagttga gaggaaaata
     1981 atcataattc taattttaat tagaaatatg atatggaaag taagcaaaac cacaaggctt
     2041 ttgctctgaa cttatttccg aaaaaaaaag tgaaactgaa agttagaatt ttcaaaataa
     2101 atattgcgag agtcaacctt cagaaaaatt ggcgagaaat caaatttgaa ttctctgaaa
     2161 atttagttaa aatacatatg ttatccttct ttgttttttg gccttagcct gccttcaagg
     2221 cgacctgagc ctatgcgggt gcctaatacc gaaacacaca taattttttt ttttaatctt
     2281 taaatactgt aacgagtcgc gagatccagc gagacccata gaatatgttg agcgctgcaa
     2341 acaatcgtat aactcttgtc caaatttttc cactctgtat ttttgcaagc agttcaacaa
     2401 atggtcatca tttttataac ccatatctat attcgaatct gacataacaa ataatactaa
     2461 gcacatcctc ccacatctct tttcattcaa gttgcgcaat tcccctaata actatggatg
     2521 gaggggggac cgagcccaaa gattattcaa aacgataacg actttttcat gcggccaacg
     2581 aaaaataaaa cttttctcat attctcctat ttcccatttc aagaattaca tccccatggg
     2641 acgttgaaat cacatcatat tagtatatgt taattgttct tttcactgga atatcgccac
     2701 gccccccttc cggtggctca ttggtttcga gaagaaggcg gagccgaagt gggttcttct
     2761 ttcttttttt tgtggtagaa gtctagcagg attaagctac tattccatta gaggatcttc
     2821 ttcttccaaa aagtgaaagg cgcgcctcta gaggatcccc gggattggcc aaaggaccca
     2881 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta
     2941 gcatgccgcc taaacgcaag acactcaatg ccgctgccga agccaatgcc cacgctgatg
     3001 gccatgctga tggcaatgct gatggtcatg tcgctaacac tgcagcaagc agcaacaacg
     3061 cccgttttgc ggacttgacc aacattgaca cacccggcct cggccctacc acgacgacgt
     3121 tacttgtcga gcccgctcgt tcgaaacgcc agagagtctc gagggcctgt gatcagtgtc
     3181 gagctgcacg tgaaaagtgt gatggaatcc agccggcttg cttcccctgt gtgtcgcagg
     3241 gccggtcgtg tacctaccag gccagtccca agaagcgagg agtccagacg ggctacatcc
     3301 gcactctcga actggctctg gcttggatgt tcgagaacgt tgcccgcagc gaggacgccc
     3361 tccacaatct tttggtccgt gatgctggcc agggcagcgc tctcctggtc ggcaaagact
     3421 cgcctgctgc agaacgcctg catgcaagat gggcgacgag tcgagtcaac aaaagcatca
     3481 cccgtcttct ctcaggtcag gccgcacaag atccatctga agacggccaa tccccgtccg
     3541 aagacataaa tgtccaagat gccggggcaa agacatccga cttccctcat gcgcctcact
     3601 tgactttctc ggcgcctaag tctagtacag ctgagacacg cactctacca ggcccggtcc
     3661 gaccacctat ttcggcaaac accctggaaa acaaccttca gccagatggt accgggatag
     3721 ggaagctacc acccaatcat tggcgcctgc tggatatcta cttttcctac acgcattctt
     3781 ggctccctat cctcgagaag aaagacatgt accaagcatt gtaccagtac tctgaacaag
     3841 gctcgttgct tccctctgcg aatgtcgagt ctggcgttca tgccgagctc tggagcgcgc
     3901 tcgccctggc gtccttccag gctgctgcta ctgctgcatc gagtgctacg ggtccagctt
     3961 cagctgctca tggccatgac aatgccatca atccttcacc tgcagacata tctgacacag
     4021 cccgaaagct catacctttg gaaagcgggc cgttccaggt tcagcactgc agagcgttgc
     4081 tgcttctttg tctcgtaagc cttgggcggg atgattggga gtctgcttgg ttgctggttg
     4141 gctttgcggt ccgcgtccta cttgttgttc gcacccagtt gcctcctgat gatgaccggc
     4201 cacgaccaag aatgcgtgcg ctgctcgtcg cgtgcttcat cgtggatacc attgtgtcta
     4261 tgagacacaa cgtgccggcc catctcaagc cagacgacat tgcggatctg ccgttacctg
     4321 aagacggtca agatcaatgg gagccgtgga caccatgtga gggcttaggc ggtgaacaca
     4381 ccatgctgca aatgttgagg aacccggcat accctttaag cacattcaac cacctatatg
     4441 gcgtgaccaa gctggttgct ttggagcttc tgccaagaat acgaacatct tcacagaacg
     4501 ctcccttgga gttcaggtcg cggttgcagc aggtaatcgg ccacaattct cccttcagcg
     4561 tctttgtcct ttcccaggat acagcatcgg cttttgtgcc tactgcatac cttacccgta
     4621 ccgtttattt atgggcagct gccttttctg agcctctcaa cgaacactac tcgcatcttc
     4681 tgatcgaaac tcttgatcag tatcagaagc ggtttggtac atatgcaatc ccacctctga
     4741 tcccttctct tctagactcc cttcttgctt taaagaaaca atcacattct tcagagcggc
     4801 atcgaaggca cttggaagag cttttccccg cctactcctc catttggcct cggggaggcc
     4861 gacacagcaa tactggcctc caacccatac gacaacttga gcttccgccg actgcgactg
     4921 ccactgcaag tatcatgccc catgtcatgg aacagcccct gtcaacgtca ataaatccgg
     4981 tcaatgatcg gtttaatgga ataccgaatc ctacccccta caatagcgat gcggccctag
     5041 acgcaattac tcaaaccaat gactacggat cagtcaatac ccatggcatt ctcagtacat
     5101 atccaccacc tgccacccac ttgaacgagg cctcggtggc ccttgcgcct ggaggtgctc
     5161 ctcccagacc acccccgcca tacgttgata gtacaacgaa ccatcctcct taccatagta
     5221 acctcgtccc catggccaat ttcggatatt cgactgtaga ctatgatgcg atggtggatg
     5281 atctggcatc gatcgagtac acggatgcgg ttgacgttga tccgcagttc atgacgaacc
     5341 tcgggtttgt cccagggtgt aattttagcg atatcaacac gtatgagcaa taggctagcg
     5401 tcgacggtac cgctgtctca tcctactttc acctagttaa ctgcttgtct taaaatctat
     5461 gcttctcttt agtatctaaa attttcctag aagcttacaa gtatataaat ggtctcttct
     5521 caataaaggt tgtatattta ttcatcttat tgaatctgcc atttcctcgt ttttgcgagt
     5581 ttatatacct tccaattttc tttctattgt attttcaact tctaatttta attcagggaa
     5641 actgcttcaa cgcatcatgg tctcaaaggg tgaagaagat aacatggcaa ttattaaaga
     5701 gtttatgcgt ttcaaggtgc atatggaggg atctgtcaat gggcatgagt ttgaaattga
     5761 aggtgaagga gaaggccgac catatgaggg aacacaaacc gcaaaactaa aggtaagttt
     5821 aaacatatat atactaacta accctgatta tttaaatttt caggtaacta aaggcggacc
     5881 attaccattc gcctgggaca tcctctctcc acagttcatg tatggaagta aagcttatgt
     5941 taaacatccg gcagatatac cagattattt gaaactttca ttcccggagg gttttaagtg
     6001 ggaacgcgta atgaattttg aagacggagg agttgttaca gtgacgcaag actcaaggta
     6061 agtttaaaca gttcggtacg aagttcctat actttctaga gaataggaac ttctaactaa
     6121 ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc
     6181 gaggaacgaa tttcccctcg gatggacctg ttatgcagaa gaagactatg ggatgggaag
     6241 cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc
     6301 ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa
     6361 ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc
     6421 agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt
     6481 acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg
     6541 aattgtataa gtaagagctc cgcatcggcc gctgtcatca gatcgccatc tcgcgcccgt
     6601 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     6661 tcccaccccc tatttttgtt attatcaaaa aaacttcttc ttaatttctt tgttttttag
     6721 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     6781 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     6841 tctacacaat gttctgtgta cacttcttat gtttttttta cttctgataa attttttttg
     6901 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     6961 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     7021 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     7081 tttcctgctt ttgctttttg ggggtttccc ctattgtttg tcaagagttt cgaggacggc
     7141 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     7201 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     7261 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     7321 tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     7381 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     7441 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     7501 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7561 atgcgtaagg agaaaatacc gcatcaggcg gccttaatta agggcctcgt gatacgccta
     7621 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7681 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7741 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7801 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7861 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7921 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7981 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8041 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8101 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8161 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8221 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8281 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8341 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8401 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8461 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8521 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8581 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8641 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8701 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8761 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8821 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8881 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8941 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     9001 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9061 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9181 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     9241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9601 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9661 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9721 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9781 agcggataac aatttcacac aggaaacagc tgggggtggg gggggggttg ggggtggggt
     9841 gttgttgggg tgtgttttgt gt
//
