LOCUS       sequence-120563-        8427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4c-QS-SL2-mcherry3'UTR-unc-54.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW09
FEATURES             Location/Qualifiers
     source          1..8427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(4645..4678)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(5946..5968)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6068..6087
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6169..6187)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6255..6359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6360..6428,6429..7220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6578..6597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7391..7979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7880..7899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8133..8150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8267..8288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8303..8308,8309..8326,8327..8333)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8341..8357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8346..8368
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gcctgactgg
       61 tgatccatct cgaatttctc gtttcaaatt agtcgttgct atatatattg tcttatgtgt
      121 ggaaaacgaa taagaaaaat tagagaaaga tgagagagca ggggcagaat gaaaatttgc
      181 atggaaattg aagaagatca ctgctttttc cgatgaatga tgactaaata gaatgagttg
      241 agaggaaaat aatcataatt ctaattttaa ttagaaatat gatatggaaa gtaagcaaaa
      301 ccacaaggct tttgctctga acttatttcc gaaaaaaaaa gtgaaactga aagttagaat
      361 tttcaaaata aatattgcga gagtcaacct tcagaaaaat tggcgagaaa tcaaatttga
      421 attctctgaa aatttagtta aaatacatat gttatccttc tttgtttttt ggccttagcc
      481 tgccttcaag gcgacctgag cctatgcggg tgcctaatac cgaaacacac ataatttttt
      541 tttttaatct ttaaatactg taacgagtcg cgagatccag cgagacccat agaatatgtt
      601 gagcgctgca aacaatcgta taactcttgt ccaaattttt ccactctgta tttttgcaag
      661 cagttcaaca aatggtcatc atttttataa cccatatcta tattcgaatc tgacataaca
      721 aataatacta agcacatcct cccacatctc ttttcattca agttgcgcaa ttcccctaat
      781 aactatggat ggagggggga ccgagcccaa agattattca aaacgataac gactttttca
      841 tgcggccaac gaaaaataaa acttttctca tattctccta tttcccattt caagaattac
      901 atccccatgg gacgttgaaa tcacatcata ttagtatatg ttaattgttc ttttcactgg
      961 aatatcgcca cgcccccctt ccggtggctc attggtttcg agaagaaggc ggagccgaag
     1021 tgggttcttc tttctttttt ttgtggtaga agtctagcag gattaagcta ctattccatt
     1081 agaggatctt cttcttccaa aaagtgaaag gcgcgcctct agaggatccc cgggattggc
     1141 caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg
     1201 acccttggct agcatgaaca ccatcccggc acgccatgtc ggggatgtcg ccgcccgcga
     1261 tcctctacct ctaccgcaca tatcatcctc cgtcgccagc ggcatgaagc gttccttcgc
     1321 aaccatggcc atgctctaca acgacactgg caacagcaac gatgtcggtg cccatgccag
     1381 gcgaccacca cgaaccctct ccaatagtcg gagcacttcc gcccacagag tacctctagg
     1441 ctcttggtcg gcgcccaatt ccccgccccg ccgtgcgctg ccgcatcatc ccatcaccgc
     1501 cagcttcgat cccgatgctt ccattgttat cgccggcatt cgtggcgctg gcaagtctac
     1561 gctggccatc atggcatcta ccgccatgaa gcgcaagata gtcgacctgg aatccgagtt
     1621 ccatcatctt actggcttgt ctagttccag ctacaagaag acacacggcc cggtcgacta
     1681 tgggaggcgc cagatcgcca tcttgcagaa catcttgaat ctgcacagga cccgcgccat
     1741 tctcgtctgc tcttggctgg agcgggatgt gcaggccatg ttgcaggatt tcagcgtgtc
     1801 taatcctgtc atttacgttc tgcgcgatgc caaagctatc gaggcccatc tgaagggata
     1861 cgacaagtcc aaagttggca ccctccttga tgccaccagt actgtccttc gccgctgcac
     1921 ccgtttcgag ttcttcaacg tctccgaaga gaacctggac acccactccg cttcaacatc
     1981 accacctgct gttccggacc agcggcatac cgcgccgtat ctaacgctta aacgagccga
     2041 gcgccacttc ctcaaatttc tctccttgat tctacccaag gggaccatac cttttgtcga
     2101 gtccgccttt cccctggctt ccgtccccgt tgaacagcgc cgctttacct acgccctcgc
     2161 cttgcctgta tctgccttgc tcgacaaagg cgtcgatatc caagagcttg atgtcggtgt
     2221 agacgcaatc gagatcattg tagacgatct tgcaacgagc gaatccggcc caacgagccc
     2281 cttgggtctt gcgccccacc gagcgagcga gatcagtcgt gttgtaggcg aaatcaggag
     2341 ggacacagtg atccccatca ttctgcacgt ggtctttcca gaaagagcgc tatatgaaga
     2401 agctctgctc gcgctttaca tgacttacct gaaccatgcc ttaaggcttg cgccagatta
     2461 tcttacggtc gatctggggc tcgattctgg cttgcttggg caactaacca ccgttcaagg
     2521 aaccaccaag gtcatcggca ataaacaact tgcagaggtc aattcgccgc gctgggggga
     2581 tccatcttgg ttacaagcct atgaaaaggc ccagaataca ggatgcgact tggtaaggtt
     2641 gaccagaccg gcttcaaatc cccgggacaa cacagacatt cggcagttcc acgttgctgt
     2701 agaggccgtc gggggtccaa ggctcccatt tattgcttac aacacaggac gcctaggtcg
     2761 gacatcgatg tgttttaacg agatcctgac tccagttaca ccagtgccta ccaaggagga
     2821 tgcaatcggg ctccgcaatc cagcccatcg ctatctccag cctccgctca cggctctgga
     2881 agcaacacag gctctctact cggcatttgt ccacgaccca atgaagctgt atgtctttgg
     2941 cgcaaatgtg ggatatagct tgtccccagc catgcacaat gccgcactca aggcctgtgg
     3001 cattccacac cattacaagc ccctttccac agcaaacatc gggactttgc gcgaggttat
     3061 cagcgatccg cagtttgctg gagcctcggt cggcctgccg tttaaggtgg aaatcatcag
     3121 cctcacacac tcgctgagcc ggcacgcgaa agccatcgga gccgtcaaca ccttgattcc
     3181 ggtacgacac cttaccgcgg acggtggaat accggacgag gtgtccatgt tcaacaatat
     3241 cagccaagcc ggcgctgtca gagctctcta cggcgagaac acggattgga ttggtatccg
     3301 agcctgcctt cgccgcggtt tatcgcccgc caatgccgtg agatcaacaa gcactggtct
     3361 tgtcatcggc gctggcggaa tggctagggc agctgtctat gccatgcttc aactgggagt
     3421 caagaagatt ttgatcttta accgaacatt tgctaatgcc gagaagctgg ttctacactt
     3481 cgagaacctg ttggtcagag acgcattgcc tctgttgagc acagggccaa gatcccacga
     3541 caacacctgt tttcacatca ttcgatctcg agacgatccg ctcccagaaa acttcaaaaa
     3601 cccgaccatg atcgtttcct gcataccgac acacacagtg gacaacaccc ctgaccctga
     3661 atttactgtg cctttgcact ggctcgacaa ccccactggc ggcattgtac tagaactcga
     3721 ctacaaatgt ctcacatcac ccttgctcga acaaacacga cgcgaggctc acagaggctg
     3781 ggtcgcaatg gatggacttg acctcttgcc agaacaaggg tttgcccaat ttgaactgtt
     3841 caccgggcgg cgagcacctc gtcgcttgat gaggcgcgag gttttgcgag catacccaga
     3901 tgatcaagca aaatctcata ccgcgcagtt acagcctcgc ctcaacggaa ttgcaacgca
     3961 aatatcttga ggtaccgctg tctcatccta ctttcaccta gttaactgct tgtcttaaaa
     4021 tctatgcttc tctttagtat ctaaaatttt cctagaagct tacaagtata taaatggtct
     4081 cttctcaata aaggttgtat atttattcat cttattgaat ctgccatttc ctcgtttttg
     4141 cgagtttata taccttccaa ttttctttct attgtatttt caacttctaa ttttaattca
     4201 gggaaactgc ttcaacgcat catggtctca aagggtgaag aagataacat ggcaattatt
     4261 aaagagttta tgcgtttcaa ggtgcatatg gagggatctg tcaatgggca tgagtttgaa
     4321 attgaaggtg aaggagaagg ccgaccatat gagggaacac aaaccgcaaa actaaaggta
     4381 agtttaaaca tatatatact aactaaccct gattatttaa attttcaggt aactaaaggc
     4441 ggaccattac cattcgcctg ggacatcctc tctccacagt tcatgtatgg aagtaaagct
     4501 tatgttaaac atccggcaga tataccagat tatttgaaac tttcattccc ggagggtttt
     4561 aagtgggaac gcgtaatgaa ttttgaagac ggaggagttg ttacagtgac gcaagactca
     4621 aggtaagttt aaacagttcg gtacgaagtt cctatacttt ctagagaata ggaacttcta
     4681 actaaccata catatttaaa ttttcagcct ccaagatgga gaatttattt ataaagtcaa
     4741 acttcgagga acgaatttcc cctcggatgg acctgttatg cagaagaaga ctatgggatg
     4801 ggaagcttca agtgaaagaa tgtaccctga agacggtgct cttaagggag agattaaaca
     4861 acgtcttaaa ttgaaagatg gaggacatta cgatgctgag gtaagtttaa acatgatttt
     4921 actaactaac taatctgatt taaattttca ggtgaagaca acttacaaag ccaaaaaacc
     4981 agttcagctg ccaggagcgt acaatgttaa tattaaactg gatatcacct cccacaacga
     5041 ggattacact atcgttgagc aatatgaaag agctgaaggg cggcactcga caggtggcat
     5101 ggatgaattg tataagtaag agctccgcat cggccgctgt catcagatcg ccatctcgcg
     5161 cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc
     5221 tgtgctccca ccccctattt ttgttattat caaaaaaact tcttcttaat ttctttgttt
     5281 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     5341 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     5401 caaaatctac acaatgttct gtgtacactt cttatgtttt ttttacttct gataaatttt
     5461 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     5521 ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct
     5581 catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa
     5641 ttaattttcc tgcttttgct ttttgggggt ttcccctatt gtttgtcaag agtttcgagg
     5701 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     5761 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     5821 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     5881 ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     5941 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6001 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     6061 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     6121 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aattaagggc ctcgtgatac
     6181 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7501 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     7801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8161 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8281 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8341 ttgtgagcgg ataacaattt cacacaggaa acagctgggg gtgggggggg ggttgggggt
     8401 ggggtgttgt tggggtgtgt tttgtgt
//
