LOCUS       sequence-120564-        4688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pquas-Delta-pes10-GFP-3'UTR-unc-54.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW12
FEATURES             Location/Qualifiers
     source          1..4688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(488..516)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1264..1284
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2258..2280)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2380..2399
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2481..2499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2567..2671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2672..2740,2741..3532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2890..2909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3703..4291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4192..4211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4445..4462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4579..4600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4615..4620,4621..4638,4639..4645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4653..4669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4658..4680
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4677..4688,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4677..4688,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg gatccgggta atcgcttatc ctcggataaa
       61 caattatcct cacgggtaat cgcttatccg ctcgggtaat cgcttatcct cgggtaatcg
      121 cttatcctta agcttgatat ccctgcagga tcgatttttt gcaaattacg agcgttgtag
      181 ggggcggagc gataggtcct ataggttttg gtatatcatc attcattcat tcattggtac
      241 attcatttac ccaccttcct ctttctgagc ttctctggag ttctgtgctt cctttttccc
      301 ttatctttat actgtaattt ttaactttca gggcgcgcct ctagaggatc cccgggattg
      361 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
      421 ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg agtaaaggag aagaactttt
      481 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      541 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      601 cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc
      661 tgattattta aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt
      721 ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg
      781 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt
      841 taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt
      901 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      961 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1021 agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta
     1081 atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca
     1141 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     1201 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1261 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1321 caaatagcat tcgtagaatt ccaactgagc gccggtcgct accattacca acttgtctgg
     1381 tgtcaaaaat aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta
     1441 acgagtaata tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt
     1501 actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt
     1561 atcaaaaaaa cttcttctta atttctttgt tttttagctt cttttaagtc acctctaaca
     1621 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     1681 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     1741 ttcttatgtt ttttttactt ctgataaatt ttttttgaaa catcatagaa aaaaccgcac
     1801 acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca
     1861 cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt
     1921 ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg
     1981 gtttccccta ttgtttgtca agagtttcga ggacggcgtt tttcttgcta aaatcacaag
     2041 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
     2101 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat
     2161 gacagaatac attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc
     2221 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2281 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2341 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2401 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2461 tcaggcggcc ttaattaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     2521 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3361 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3841 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4081 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4321 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4381 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4441 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4501 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4561 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4621 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4681 aaacagct
//
