LOCUS       sequence-120565-        9863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and 
            SacI sites in QF are removed for the convenience of cloning, most 
            sites are compatible with pPD95.77).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW17
FEATURES             Location/Qualifiers
     source          1..9863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(6081..6114)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7382..7404)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7504..7523
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7605..7623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7691..7795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7796..7864,7865..8656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8014..8033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8827..9415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9316..9335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9569..9586
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9703..9724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9739..9744,9745..9762,9763..9769)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9777..9793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9782..9804
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgca gctctgagac catgttgatg ggaaattata
       61 tttgaaaact tttgaataaa gttgtataat tgtttttatt ctttaaccta tttatgaatg
      121 ctgtcccgga actgggatat aatttctaac ttgacacaat tattccacct ctgtcttcaa
      181 ggcgacctac acctacctcg cgtctaggtc caacagaaac actgttgaat gaaatcctcg
      241 aattgcaaca atataggatg ctcaggagac tataattcaa aaaacccaac ccaaattagc
      301 ataagcttcc ccaaattgga acagtgaaat actcaatcaa tcactctggc ggttttctcc
      361 acaaagaaca cgaaattgga aaaaggaaaa gattgatttg tttgaaggcg tgaggaaatc
      421 gaaaaaaaat tgtttcaaaa caaatgaaat attttaacga ataaacccag gaatgccact
      481 ttttggtgcc cgcctacaaa aaagtcaact gttcttaaac tcctacatag ttgccctctc
      541 taacgaatgt tgcttttttt aaattttcat tgaatttgat tatcgtctgt atgcgcctac
      601 tctatcagtg gaaaacatgc ccatttccca caaatttact cgtttggagc tcaaaatgtt
      661 aaatggggca tcagggaatt ggcatctcac agaaagtggc ggatgagtta tatgtaataa
      721 taatggtggc tgaagtggaa accgcaaaaa aaggatgtgt atttgagatt ttgcaacgga
      781 atttgaatca gatacacaaa atgagagaaa tttatagatt ctttaaaatc cacctgaagt
      841 cggcaaattt ttgaaaggtc tgttcgttca gtttttcata agttttatga attttgattt
      901 actactgaag ttcgagaaaa gccctttaac cataccaata agaaattatg ttaactttac
      961 aagaatatta acaaaaaatt attaaaaaac tgtgttcccc tggcattatg atatttccac
     1021 cgtcagttct ctattaaaac ttcaactcta cacagtttca actctcaaat gagctgtcca
     1081 tcttttgcct tcaggaaatt atgctaatct gctgatttcg cgcactttca ccacactatt
     1141 tccaaataga aggaggggtg gtgcaacaca cgagacactg caaaaaggtt cagtgaagat
     1201 ttatctactt gtgagatgag agcaggaggg gtgggagaag tgtgatgatt gacgggatga
     1261 caagaacagt gatgtggtgt cgaaatggaa gatgcgctaa tttgtggagt tttatgaaat
     1321 ttgaataatt acatttataa catctactag ttaggcgtct gtgtaggtat tgcctgcctg
     1381 cctacaaggc atatgtacga ggcaggttaa acctcctaga aagctacggt actttcaaac
     1441 cacataacat ttatactggt gtcttttcgc gtcttcttct tgaacatgta gaccctcaaa
     1501 aagctaagtt ttacactgtg tagtttgctt tgaaatctac aaaaattgga ggcacgcctt
     1561 ctggactttg ccgacacgcc tactttctac ctaccaactg cctacttcca cagtctagcg
     1621 tgaactccaa taccatttta aaaattgttt tatctcaaat tttcaatttt taaatccaat
     1681 ttcccagttc taaaaaaaaa attttttttt taatttaaaa actctcatcc tttttaatgc
     1741 tcgttcccat tcggcaagac gtgtaccaaa cacaaaacaa attgactggt gatccatctc
     1801 gaatttctcg tttcaaatta gtcgttgcta tatatattgt cttatgtgtg gaaaacgaat
     1861 aagaaaaatt agagaaagat gagagagcag gggcagaatg aaaatttgca tggaaattga
     1921 agaagatcac tgctttttcc gatgaatgat gactaaatag aatgagttga gaggaaaata
     1981 atcataattc taattttaat tagaaatatg atatggaaag taagcaaaac cacaaggctt
     2041 ttgctctgaa cttatttccg aaaaaaaaag tgaaactgaa agttagaatt ttcaaaataa
     2101 atattgcgag agtcaacctt cagaaaaatt ggcgagaaat caaatttgaa ttctctgaaa
     2161 atttagttaa aatacatatg ttatccttct ttgttttttg gccttagcct gccttcaagg
     2221 cgacctgagc ctatgcgggt gcctaatacc gaaacacaca taattttttt ttttaatctt
     2281 taaatactgt aacgagtcgc gagatccagc gagacccata gaatatgttg agcgctgcaa
     2341 acaatcgtat aactcttgtc caaatttttc cactctgtat ttttgcaagc agttcaacaa
     2401 atggtcatca tttttataac ccatatctat attcgaatct gacataacaa ataatactaa
     2461 gcacatcctc ccacatctct tttcattcaa gttgcgcaat tcccctaata actatggatg
     2521 gaggggggac cgagcccaaa gattattcaa aacgataacg actttttcat gcggccaacg
     2581 aaaaataaaa cttttctcat attctcctat ttcccatttc aagaattaca tccccatggg
     2641 acgttgaaat cacatcatat tagtatatgt taattgttct tttcactgga atatcgccac
     2701 gccccccttc cggtggctca ttggtttcga gaagaaggcg gagccgaagt gggttcttct
     2761 ttcttttttt tgtggtagaa gtctagcagg attaagctac tattccatta gaggatcttc
     2821 ttcttccaaa aagtgaaagg cgcgcctcta gaggatcccc gggattggcc aaaggaccca
     2881 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta
     2941 gccatgccac caaaacgcaa gacactcaat gccgctgccg aagccaatgc ccacgctgat
     3001 ggccatgctg atggcaatgc tgatggtcat gtcgctaaca ctgcagcaag cagcaacaac
     3061 gcccgttttg cggacttgac caacattgac acacccggcc tcggccctac cacgacgacg
     3121 ttacttgtcg agcccgctcg ttcgaaacgc cagagagtct cgagggcctg tgatcagtgt
     3181 cgagctgcac gtgaaaagtg tgatggaatc cagccggctt gcttcccctg tgtgtcgcag
     3241 ggccggtcgt gtacctacca ggccagtccc aagaagcgag gagtccagac gggctacatc
     3301 cgcactctcg aactggctct ggcttggatg ttcgagaacg ttgcccgcag cgaggacgcc
     3361 ctccacaatc ttttggtccg tgatgctggc cagggcagcg ctctcctggt cggcaaagac
     3421 tcgcctgctg cagaacgcct ccatgcaaga tgggcgacga gtcgagtcaa caaaagcatc
     3481 acccgtcttc tctcaggtca ggccgcacaa gatccatctg aagacggcca atccccgtcc
     3541 gaagacataa atgtccaaga tgccggggca aagacatccg acttccctca tgcgcctcac
     3601 ttgactttct cggcgcctaa gtctagtaca gctgagacac gcactctacc aggcccggtc
     3661 cgaccaccta tttcggcaaa caccctggaa aacaaccttc agccagatgg aaccgggata
     3721 gggaagctac cacccaatca ttggcgcctg ctggatatct acttttccta cacgcattct
     3781 tggctcccta tcctcgagaa gaaagacatg taccaagcat tgtaccagta ctctgaacaa
     3841 ggctcgttgc ttccctctgc gaatgtcgag tctggcgttc atgccgagct ttggagcgcg
     3901 ctcgccctgg cgtccttcca ggctgctgct actgctgcat cgagtgctac gggtccagct
     3961 tcagctgctc atggccatga caatgccatc aatccttcac ctgcagacat atctgacaca
     4021 gcccgaaagc tcataccttt ggaaagcggg ccgttccagg ttcagcactg cagagcgttg
     4081 ctgcttcttt gtctcgtaag ccttgggcgg gatgattggg agtctgcttg gttgctggtt
     4141 ggctttgcgg tccgcgtcct acttgttgtt cgcacccagt tgcctcctga tgatgaccgg
     4201 ccacgaccaa gaatgcgtgc gctgctcgtc gcgtgcttca tcgtggatac cattgtgtct
     4261 atgagacaca acgtgccggc ccatctcaag ccagacgaca ttgcggatct gccgttacct
     4321 gaagacggtc aagatcaatg ggagccgtgg acaccatgtg agggcttagg cggtgaacac
     4381 accatgctgc aaatgttgag gaacccggca taccctttaa gcacattcaa ccacctatat
     4441 ggcgtgacca agctggttgc tttggagctt ctgccaagaa tacgaacatc ttcacagaac
     4501 gctcccttgg agttcaggtc gcggttgcag caggtaatcg gccacaattc tcccttcagc
     4561 gtctttgtcc tttcccagga tacagcatcg gcttttgtgc ctactgcata ccttacccgt
     4621 accgtttatt tatgggcagc tgccttttct gagcctctca acgaacacta ctcgcatctt
     4681 ctgatcgaaa ctcttgatca gtatcagaag cggtttggta catatgcaat cccacctctg
     4741 atcccttctc ttctagactc ccttcttgct ttaaagaaac aatcacattc ttcagagcgg
     4801 catcgaaggc acttggaaga gcttttcccc gcctactcct ccatttggcc tcggggaggc
     4861 cgacacagca atactggcct ccaacccata cgacaacttg agcttccgcc gactgcgact
     4921 gccactgcaa gtatcatgcc ccatgtcatg gaacagcccc tgtcaacgtc aataaatccg
     4981 gtcaatgatc ggtttaatgg aataccgaat cctaccccct acaatagcga tgcggcccta
     5041 gacgcaatta ctcaaaccaa tgactacgga tcagtcaata cccatggcat tctcagtaca
     5101 tatccaccac ctgccaccca cttgaacgag gcctcggtgg cccttgcgcc tggaggtgct
     5161 cctcccagac cacccccgcc atacgttgat agtacaacga accatcctcc ttaccatagt
     5221 aacctcgtcc ccatggccaa tttcggatat tcgactgtag actatgatgc gatggtggat
     5281 gatctggcat cgatcgagta cacggatgcg gttgacgttg atccgcagtt catgacgaac
     5341 ctcgggtttg tcccagggtg taattttagc gatatcaaca cgtatgagca ataggctagc
     5401 gtcgacggta ccgctgtctc atcctacttt cacctagtta actgcttgtc ttaaaatcta
     5461 tgcttctctt tagtatctaa aattttccta gaagcttaca agtatataaa tggtctcttc
     5521 tcaataaagg ttgtatattt attcatctta ttgaatctgc catttcctcg tttttgcgag
     5581 tttatatacc ttccaatttt ctttctattg tattttcaac ttctaatttt aattcaggga
     5641 aactgcttca acgcatcatg gtctcaaagg gtgaagaaga taacatggca attattaaag
     5701 agtttatgcg tttcaaggtg catatggagg gatctgtcaa tgggcatgag tttgaaattg
     5761 aaggtgaagg agaaggccga ccatatgagg gaacacaaac cgcaaaacta aaggtaagtt
     5821 taaacatata tatactaact aaccctgatt atttaaattt tcaggtaact aaaggcggac
     5881 cattaccatt cgcctgggac atcctctctc cacagttcat gtatggaagt aaagcttatg
     5941 ttaaacatcc ggcagatata ccagattatt tgaaactttc attcccggag ggttttaagt
     6001 gggaacgcgt aatgaatttt gaagacggag gagttgttac agtgacgcaa gactcaaggt
     6061 aagtttaaac agttcggtac gaagttccta tactttctag agaataggaa cttctaacta
     6121 accatacata tttaaatttt cagcctccaa gatggagaat ttatttataa agtcaaactt
     6181 cgaggaacga atttcccctc ggatggacct gttatgcaga agaagactat gggatgggaa
     6241 gcttcaagtg aaagaatgta ccctgaagac ggtgctctta agggagagat taaacaacgt
     6301 cttaaattga aagatggagg acattacgat gctgaggtaa gtttaaacat gattttacta
     6361 actaactaat ctgatttaaa ttttcaggtg aagacaactt acaaagccaa aaaaccagtt
     6421 cagctgccag gagcgtacaa tgttaatatt aaactggata tcacctccca caacgaggat
     6481 tacactatcg ttgagcaata tgaaagagct gaagggcggc actcgacagg tggcatggat
     6541 gaattgtata agtaagagct ccgcatcggc cgctgtcatc agatcgccat ctcgcgcccg
     6601 tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg
     6661 ctcccacccc ctatttttgt tattatcaaa aaaacttctt cttaatttct ttgtttttta
     6721 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     6781 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     6841 atctacacaa tgttctgtgt acacttctta tgtttttttt acttctgata aatttttttt
     6901 gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat
     6961 gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc
     7021 gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa
     7081 ttttcctgct tttgcttttt gggggtttcc cctattgttt gtcaagagtt tcgaggacgg
     7141 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     7201 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     7261 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     7321 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     7381 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7441 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7501 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7561 gatgcgtaag gagaaaatac cgcatcaggc ggccttaatt aagggcctcg tgatacgcct
     7621 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7801 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7861 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7921 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7981 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8041 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8101 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8161 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8221 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8281 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8341 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8401 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8461 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8521 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8581 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8641 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8701 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8941 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     9001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9181 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     9241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9481 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9541 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9601 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9661 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9721 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9781 gagcggataa caatttcaca caggaaacag ctgggggtgg ggggggggtt gggggtgggg
     9841 tgttgttggg gtgtgttttg tgt
//
