LOCUS       sequence-120567-        7299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW21
FEATURES             Location/Qualifiers
     source          1..7299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4818..4840)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4940..4959
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5041..5059)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5127..5231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5232..5300,5301..6092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5450..5469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6263..6851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6752..6771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7005..7022
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7139..7160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7175..7180,7181..7198,7199..7205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7213..7229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7218..7240
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gcctgactgg
       61 tgatccatct cgaatttctc gtttcaaatt agtcgttgct atatatattg tcttatgtgt
      121 ggaaaacgaa taagaaaaat tagagaaaga tgagagagca ggggcagaat gaaaatttgc
      181 atggaaattg aagaagatca ctgctttttc cgatgaatga tgactaaata gaatgagttg
      241 agaggaaaat aatcataatt ctaattttaa ttagaaatat gatatggaaa gtaagcaaaa
      301 ccacaaggct tttgctctga acttatttcc gaaaaaaaaa gtgaaactga aagttagaat
      361 tttcaaaata aatattgcga gagtcaacct tcagaaaaat tggcgagaaa tcaaatttga
      421 attctctgaa aatttagtta aaatacatat gttatccttc tttgtttttt ggccttagcc
      481 tgccttcaag gcgacctgag cctatgcggg tgcctaatac cgaaacacac ataatttttt
      541 tttttaatct ttaaatactg taacgagtcg cgagatccag cgagacccat agaatatgtt
      601 gagcgctgca aacaatcgta taactcttgt ccaaattttt ccactctgta tttttgcaag
      661 cagttcaaca aatggtcatc atttttataa cccatatcta tattcgaatc tgacataaca
      721 aataatacta agcacatcct cccacatctc ttttcattca agttgcgcaa ttcccctaat
      781 aactatggat ggagggggga ccgagcccaa agattattca aaacgataac gactttttca
      841 tgcggccaac gaaaaataaa acttttctca tattctccta tttcccattt caagaattac
      901 atccccatgg gacgttgaaa tcacatcata ttagtatatg ttaattgttc ttttcactgg
      961 aatatcgcca cgcccccctt ccggtggctc attggtttcg agaagaaggc ggagccgaag
     1021 tgggttcttc tttctttttt ttgtggtaga agtctagcag gattaagcta ctattccatt
     1081 agaggatctt cttcttccaa aaagtgaaag gcgcgcctct agaggatccc cgggattggc
     1141 caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg
     1201 acccttggct agcatgaaca ccatcccggc acgccatgtc ggggatgtcg ccgcccgcga
     1261 tcctctacct ctaccgcaca tatcatcctc cgtcgccagc ggcatgaagc gttccttcgc
     1321 aaccatggcc atgctctaca acgacactgg caacagcaac gatgtcggtg cccatgccag
     1381 gcgaccacca cgaaccctct ccaatagtcg gagcacttcc gcccacagag tacctctagg
     1441 ctcttggtcg gcgcccaatt ccccgccccg ccgtgcgctg ccgcatcatc ccatcaccgc
     1501 cagcttcgat cccgatgctt ccattgttat cgccggcatt cgtggcgctg gcaagtctac
     1561 gctggccatc atggcatcta ccgccatgaa gcgcaagata gtcgacctgg aatccgagtt
     1621 ccatcatctt actggcttgt ctagttccag ctacaagaag acacacggcc cggtcgacta
     1681 tgggaggcgc cagatcgcca tcttgcagaa catcttgaat ctgcacagga cccgcgccat
     1741 tctcgtctgc tcttggctgg agcgggatgt gcaggccatg ttgcaggatt tcagcgtgtc
     1801 taatcctgtc atttacgttc tgcgcgatgc caaagctatc gaggcccatc tgaagggata
     1861 cgacaagtcc aaagttggca ccctccttga tgccaccagt actgtccttc gccgctgcac
     1921 ccgtttcgag ttcttcaacg tctccgaaga gaacctggac acccactccg cttcaacatc
     1981 accacctgct gttccggacc agcggcatac cgcgccgtat ctaacgctta aacgagccga
     2041 gcgccacttc ctcaaatttc tctccttgat tctacccaag gggaccatac cttttgtcga
     2101 gtccgccttt cccctggctt ccgtccccgt tgaacagcgc cgctttacct acgccctcgc
     2161 cttgcctgta tctgccttgc tcgacaaagg cgtcgatatc caagagcttg atgtcggtgt
     2221 agacgcaatc gagatcattg tagacgatct tgcaacgagc gaatccggcc caacgagccc
     2281 cttgggtctt gcgccccacc gagcgagcga gatcagtcgt gttgtaggcg aaatcaggag
     2341 ggacacagtg atccccatca ttctgcacgt ggtctttcca gaaagagcgc tatatgaaga
     2401 agctctgctc gcgctttaca tgacttacct gaaccatgcc ttaaggcttg cgccagatta
     2461 tcttacggtc gatctggggc tcgattctgg cttgcttggg caactaacca ccgttcaagg
     2521 aaccaccaag gtcatcggca ataaacaact tgcagaggtc aattcgccgc gctgggggga
     2581 tccatcttgg ttacaagcct atgaaaaggc ccagaataca ggatgcgact tggtaaggtt
     2641 gaccagaccg gcttcaaatc cccgggacaa cacagacatt cggcagttcc acgttgctgt
     2701 agaggccgtc gggggtccaa ggctcccatt tattgcttac aacacaggac gcctaggtcg
     2761 gacatcgatg tgttttaacg agatcctgac tccagttaca ccagtgccta ccaaggagga
     2821 tgcaatcggg ctccgcaatc cagcccatcg ctatctccag cctccgctca cggctctgga
     2881 agcaacacag gctctctact cggcatttgt ccacgaccca atgaagctgt atgtctttgg
     2941 cgcaaatgtg ggatatagct tgtccccagc catgcacaat gccgcactca aggcctgtgg
     3001 cattccacac cattacaagc ccctttccac agcaaacatc gggactttgc gcgaggttat
     3061 cagcgatccg cagtttgctg gagcctcggt cggcctgccg tttaaggtgg aaatcatcag
     3121 cctcacacac tcgctgagcc ggcacgcgaa agccatcgga gccgtcaaca ccttgattcc
     3181 ggtacgacac cttaccgcgg acggtggaat accggacgag gtgtccatgt tcaacaatat
     3241 cagccaagcc ggcgctgtca gagctctcta cggcgagaac acggattgga ttggtatccg
     3301 agcctgcctt cgccgcggtt tatcgcccgc caatgccgtg agatcaacaa gcactggtct
     3361 tgtcatcggc gctggcggaa tggctagggc agctgtctat gccatgcttc aactgggagt
     3421 caagaagatt ttgatcttta accgaacatt tgctaatgcc gagaagctgg ttctacactt
     3481 cgagaacctg ttggtcagag acgcattgcc tctgttgagc acagggccaa gatcccacga
     3541 caacacctgt tttcacatca ttcgatctcg agacgatccg ctcccagaaa acttcaaaaa
     3601 cccgaccatg atcgtttcct gcataccgac acacacagtg gacaacaccc ctgaccctga
     3661 atttactgtg cctttgcact ggctcgacaa ccccactggc ggcattgtac tagaactcga
     3721 ctacaaatgt ctcacatcac ccttgctcga acaaacacga cgcgaggctc acagaggctg
     3781 ggtcgcaatg gatggacttg acctcttgcc agaacaaggg tttgcccaat ttgaactgtt
     3841 caccgggcgg cgagcacctc gtcgcttgat gaggcgcgag gttttgcgag catacccaga
     3901 tgatcaagca aaatctcata ccgcgcagtt acagcctcgc ctcaacggaa ttgcaacgca
     3961 aatatcttga ggtaccatgg tattgatatc tgagctccgc atcggccgct gtcatcagat
     4021 cgccatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     4081 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaaa cttcttctta
     4141 atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa
     4201 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     4261 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttttactt
     4321 ctgataaatt ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     4381 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     4441 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     4501 aagtttatga aattaatttt cctgcttttg ctttttgggg gtttccccta ttgtttgtca
     4561 agagtttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     4621 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     4681 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     4741 taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc
     4801 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4861 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4921 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     4981 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaattaagg
     5041 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5221 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5281 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5341 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5401 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5461 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5521 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5581 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5641 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5701 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5761 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5821 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5881 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5941 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6001 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6061 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     6661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6901 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6961 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7021 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7081 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7141 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7201 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctgg gggtgggggg
     7261 ggggttgggg gtggggtgtt gttggggtgt gttttgtgt
//
