LOCUS       sequence-120568-        8653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q 
            intersectional method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW52
FEATURES             Location/Qualifiers
     source          1..8653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4172..4192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(4871..4904)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(6172..6194)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6294..6313
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6395..6413)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6481..6585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6586..6654,6655..7446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6804..6823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7617..8205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8106..8125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8359..8376
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8493..8514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8529..8534,8535..8552,8553..8559)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8567..8583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8572..8594
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgacactaag tttctctgca gtgttacacg
       61 aactttttac aatctactac tactaacgaa cgtcgcttgt atgtgttgcg ctctgaaaag
      121 ttcacatctg ttgctgcctt ttgtacatta tttgagaatc tttactcatt tcgtgttctt
      181 ttatcaattt agtgaataaa ctgattcata ttgattaaca agtagttgaa caaaaagaaa
      241 cggtgtcctt ttgtgtttgc attttatctc tgtcctttgt agtctgattt taattttttc
      301 ttactctatt catctccttc tctgctccac caaaccggta tatatgacta gggcttacac
      361 agacgtgctt cattttcttt tcttgcttct tccccacaaa tgatacactg tcaatgggcg
      421 aggaagaaga gctgcttctt gggacggact cgtcgttctt tctcatcaat caccggcgat
      481 gcactcgaca caaacacgtt acaagaaaga ccctcccggg ctctttcagc ccattttgtt
      541 gaaatatgtg cttttgaaac gcaacattcg aatgatttgt cacgacagtc ggcctaatta
      601 attgactctc gaaaatgagc atacgattga gtgttaatgt gccgaagatg tagggaggag
      661 caagaaaaga gggtggaaga gatgatgagt gacagactgt cttaacagat gacctgcttg
      721 tggtggtcac ctgtcgagtt atgatctcag taattgcttc aattttccac atacgttccc
      781 aagtgtctca ttagaatata aataagacaa tattttgttc tgtgtcctgt agagaataca
      841 caattccaac tagaaacgtt tttttttcat ttttttaaat tgatttcaaa acatcttttg
      901 acctgatgtc agtgtggggg atttttttgg catttgtaat cgaaattaat gaatttttag
      961 acaatttctg aaaaatagta aggtttaaaa aaatcttaag gacggcacac actgtacaac
     1021 aatactttga ctgatggttt ttctgttttt cattacaatc ttattttttc tagtcagctt
     1081 ttttatgtga ttatttaaaa aaacaatggt cttgctttag atagtcaaga tctcacactc
     1141 ttaataaaaa aggttttaat acttcagatt ttgctttaac ttttggaaat tgccaaccgt
     1201 ttctgaaaaa tttgaagtct ttgaaaacaa ttacagcact tttgcacgtt ttgctgctat
     1261 caaatgttta atataaataa gcaaaatatt tgtgaaaaat aaaatacttc acttcatttt
     1321 ttaaattttg cttctttctc acaaatgata tattgactgt aaatgtcaga attttgaaaa
     1381 gttaaaaaaa attatgttga aaatttattt ttttttctta ctgacagact gatgtgcctt
     1441 tttcagcatc ttgaataagt tcaagttttt ttaatcgaag ctgtttccag tgtctgtcag
     1501 tgacccacac actagaacgc ctaaaataaa aatcgaaatt tttggagtat ccgaatttaa
     1561 tttggaatca aattgatgct tgaaatatat gtgagctctt aaaataataa taaaaaattg
     1621 ctacaatcat tttattaaaa tatttttcga tctaaaaatt gttcatcatt gagtaatgta
     1681 attttgataa attagacccc ccccccttct tctacaaaac tgatcaaaag gtccgaaaga
     1741 cttagaagac ccgcacaata gtagaatatt caatggccga atagtgaatg cgcgaggcgg
     1801 gtggttgctg tcttctcggt tcatataagg ctcatctgtt caatcaccaa atgatcgatg
     1861 tgtttttgtg gccttcggcg tcagtttgga gcagcacttc aacctcattg aaacctctgt
     1921 cttcctccta tctttgcctt tttttagtca agaggcggtg tgaacgacgc aatattcccg
     1981 ttggttatca ccacactaga agtttttttg agcactctta tcagggaaca attattgatg
     2041 atttattgcc aactagtggg ttggttttgc tattttttga aatttactgg tatggtcttt
     2101 tgagattttc aggcagttaa aacttactaa gactgatggt taacaaaaag ttgtaatata
     2161 caatactaac tgaagcgaaa ctttgaacga aaaatatatt aaactgttaa attttatttg
     2221 cagctagcta actaataatt tgtatgttta gtgcatttaa aaaagttttc agctatatta
     2281 taatgatagt ttgaaactat gataaaactg agtttatacg actttttgaa tacacaaaaa
     2341 aaatttaaaa attttttgaa aactttttta atgtattttt tgtagtattt tattttccct
     2401 ggcctaggaa ttatgaaaaa aagtttctag atttcaattt attccttatt ttgacaaatt
     2461 attatgaaat aattcattaa tttcattttg tacgtaaatg ggcgggacta atgagatcct
     2521 caaactcctt gtgattctct ttcctcctcg ccttcttcta ccatactcct ggacctcgct
     2581 cttcccgcgc actctcttcc ccctctttca ctcttgccac acttgccaag ggagtcataa
     2641 atcgtgcaac atgtttagta ggtgccgttt tgaaatatag atgctctttt tttctttctc
     2701 acaaaccatc ttttagttgg tctcgctcat ctattttgaa aaatgacgat ccgtgtagag
     2761 ctttgattct tcatagttat tttcttgtgt gtgcgtcggg cctcatactg ccaggtagag
     2821 tgtacgtgtt tacctaacaa gggagaggtc tatgtggcac tgcgcacttc atcgtatgca
     2881 aatttgcttt tatgatctct tccctcaggt cattgtccat cataagcatc cacacacttt
     2941 ttttttggtg ttttcttctc tttttttggg tcaccagttc acaaccaaaa atataggtgg
     3001 tcaaaacata acatttttct gtacttacag taagtggaaa tgtcgtctaa gcacacttag
     3061 aatattctaa ataaaagaga attattagaa aacaacttta aaatgtttct ggcaacgttt
     3121 tgaaagtttg agaaatttat aacaaacgtt caaatcaagt tgcaacataa ttagaatata
     3181 aaatttgtga aaaacacttt ttggtcaact tccaaaatta tcaatgatga aaaagtgact
     3241 gtttgcaact atttggcttt aaattaaaga actaattttt tattttaatt ttcactattt
     3301 ccctacaagc aacctgtaaa gtgatattgt gtgtgactat tttttctctt ttaccacaat
     3361 atttgagtat taacccccct ttagctgtct tcaattatca tttaattcag aatttcaggt
     3421 aggcgcgcct ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     3481 atactaacat aacatagaac attttcagga ggacccttgg ctagccatgg ccagcgagca
     3541 gctggagaag aaactccagg ctctcgagaa aaagctggca cagcttgagt ggaagaacca
     3601 ggcactggag aagaaactgg cacagggatc tggatctgga tctggatctg gttccggtac
     3661 ccttgagctt ccgccgactg cgactgccac tgcaagtatc atgccccatg tcatggaaca
     3721 gcccctgtca acgtcaataa atccggtcaa tgatcggttt aatggaatac cgaatcctac
     3781 cccctacaat agcgatgcgg ccctagacgc aattactcaa accaatgact acggatcagt
     3841 caatacccat ggcattctca gtacatatcc accacctgcc acccacttga acgaggcctc
     3901 ggtggccctt gcgcctggag gtgctcctcc cagaccaccc ccgccatacg ttgatagtac
     3961 aacgaaccat cctccttacc atagtaacct cgtccccatg gccaatttcg gatattcgac
     4021 tgtagactat gatgcgatgg tggatgatct ggcatcgatc gagtacacgg atgcggttga
     4081 cgttgatccg cagttcatga cgaacctcgg gtttgtccca gggtgtaatt ttagcgatat
     4141 caacacgtat gagcaaggat ctggatctat gccaaagaag aagcgtaagg tctaagggta
     4201 ccgctgtctc atcctacttt cacctagtta actgcttgtc ttaaaatcta tgcttctctt
     4261 tagtatctaa aattttccta gaagcttaca agtatataaa tggtctcttc tcaataaagg
     4321 ttgtatattt attcatctta ttgaatctgc catttcctcg tttttgcgag tttatatacc
     4381 ttccaatttt ctttctattg tattttcaac ttctaatttt aattcaggga aactgcttca
     4441 acgcatcatg gtctcaaagg gtgaagaaga taacatggca attattaaag agtttatgcg
     4501 tttcaaggtg catatggagg gatctgtcaa tgggcatgag tttgaaattg aaggtgaagg
     4561 agaaggccga ccatatgagg gaacacaaac cgcaaaacta aaggtaagtt taaacatata
     4621 tatactaact aaccctgatt atttaaattt tcaggtaact aaaggcggac cattaccatt
     4681 cgcctgggac atcctctctc cacagttcat gtatggaagt aaagcttatg ttaaacatcc
     4741 ggcagatata ccagattatt tgaaactttc attcccggag ggttttaagt gggaacgcgt
     4801 aatgaatttt gaagacggag gagttgttac agtgacgcaa gactcaaggt aagtttaaac
     4861 agttcggtac gaagttccta tactttctag agaataggaa cttctaacta accatacata
     4921 tttaaatttt cagcctccaa gatggagaat ttatttataa agtcaaactt cgaggaacga
     4981 atttcccctc ggatggacct gttatgcaga agaagactat gggatgggaa gcttcaagtg
     5041 aaagaatgta ccctgaagac ggtgctctta agggagagat taaacaacgt cttaaattga
     5101 aagatggagg acattacgat gctgaggtaa gtttaaacat gattttacta actaactaat
     5161 ctgatttaaa ttttcaggtg aagacaactt acaaagccaa aaaaccagtt cagctgccag
     5221 gagcgtacaa tgttaatatt aaactggata tcacctccca caacgaggat tacactatcg
     5281 ttgagcaata tgaaagagct gaagggcggc actcgacagg tggcatggat gaattgtata
     5341 agtaagagct ccgcatcggc cgctgtcatc agatcgccat ctcgcgcccg tgcctctgac
     5401 ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc
     5461 ctatttttgt tattatcaaa aaaacttctt cttaatttct ttgtttttta gcttctttta
     5521 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     5581 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     5641 tgttctgtgt acacttctta tgtttttttt acttctgata aatttttttt gaaacatcat
     5701 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     5761 tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt
     5821 tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct
     5881 tttgcttttt gggggtttcc cctattgttt gtcaagagtt tcgaggacgg cgtttttctt
     5941 gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt
     6001 gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg
     6061 tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt ttcctactag
     6121 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6181 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6241 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6301 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6361 gagaaaatac cgcatcaggc ggccttaatt aagggcctcg tgatacgcct atttttatag
     6421 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6481 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6541 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6601 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6661 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6721 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6781 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6841 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6901 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6961 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7021 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7081 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7141 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7201 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7261 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7321 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7381 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7441 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7741 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7981 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     8041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8281 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8341 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8401 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8461 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8521 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8581 caatttcaca caggaaacag ctgggggtgg ggggggggtt gggggtgggg tgttgttggg
     8641 gtgtgttttg tgt
//
