LOCUS       sequence-120575-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pa-G-GECO1.2
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(409..430)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(643..699)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(748..767)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(749..767)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcacctcg gcgcgggtct tgtagttgcc gtcgcccttg aagaagatgg tgcgctcctg
       61 gatgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      121 gcggctgaag cactgcacgc cgtagctgaa ggtggtcacg agggtgggcc agggcacggg
      181 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      241 accctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgacctggat
      301 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggattcgc tccctccggt
      361 accgcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      421 caggaccatg tgatcgcgct tctcgttggg gtctttcgaa agtttggact gatggctcag
      481 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      541 gtaggcgagc tgcacgccgc cgccctcgat gttgtggcgg atcttgaagt acgccttgat
      601 gccgttcttc tgctcgtcgg ccttgatata gacgttctcg agtgagctca gccgacctat
      661 agctctgact gcgtgacctg tcttattcca cttacgacgt gatgagtcga ccatggtgga
      721 tccgagctcg gtaccaagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc
      781 agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc t
//
