LOCUS       sequence-120576-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pa-G-GECO1.2
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..170
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     383..404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccaccat ggtcgactca tcacgtcgta
      121 agtggaataa gacaggtcac gcagtcagag ctataggtcg gctgagctca ctcgagaacg
      181 tctatatcaa ggccgacgag cagaagaacg gcatcaaggc gtacttcaag atccgccaca
      241 acatcgaggg cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg
      301 acggccccgt gctgctgccc gacaaccact acctgagcca tcagtccaaa ctttcgaaag
      361 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      421 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcgaatcc atggtgagca
      481 agggcgagga gctgttcacc ggggtggtgc ccatccaggt cgagctggac ggcgacgtaa
      541 acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac ggcaagctga
      601 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      661 ccttcagcta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      721 tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaagggcg
      781 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      841 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      901 acaacacgcg tgaccaactg actgaagagc agatcgcaga atttaaagag gctttctccc
      961 tatttgacaa ggacggggat gggacgataa caaccaagga gctgggggac ggtgatgcgg
     1021 tctctggggg cagaacccca caga
//
