LOCUS       sequence-120577-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 FOXM
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtactgtagc tcaggaataa actgggacca gttgatgttg tcagggccca
      241 gtgggtcctc gtccaggcca ggaaagctga tgtccagcag gatcttgctg aggctgtcat
      301 tcattgtgtc caggaccagg ccttctgtca gagaacgatt ggctgcaagg ccagaaacct
      361 gtggctccgg ggagcctggc ttggggacgt ctatatctga gggagaagag ttgccaaagg
      421 ggacggagat gaggtctaag ggttctgaac tgaggagcct ttgcggtgat tcaagggggg
      481 gagcactttg caagggagtg gtgctgagat ccatcagccc cagggggtca ggcaaggggt
      541 cagaggcacc ctggggggtt tgtactgggc tgaaatccag tccccctact ttggctgggg
      601 gcgtgagcct ccaggattca ggggttctgg ggaggacaga tttgctcggg gtggaggaga
      661 tgggcagcgt ttccttaatg ggtgtcttaa aaggtcctcc cacttcctgg gagtagctga
      721 gctgggaggc agggtcagag gagtctgctg ggaacgggag ctctgcggcc cagcgggaag
      781 tactgggccc ctctgagaag agcagctccg gctcatccac acaggg
//
