LOCUS       sequence-120578-         400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the tRNALys scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
  TITLE     A generic protocol for the expression and purification of 
            recombinant RNA in Escherichia coli using a tRNA scaffold.
  JOURNAL   Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 
            May 28.
  PUBMED    19478810
REFERENCE   2  (bases 1 to 400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2009"; volume: "4(6):947-59. doi"; pages: " 
            10.1038/nprot.2009.67. Epub 2009 May 28"
COMMENT     Sequence Label: pBSK
FEATURES             Location/Qualifiers
     source          1..400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(202..221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(203..221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(228..245)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(228..244)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(237..259)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcanataaaa aactttgtgt aatacttgta acgctgaatt cgcccggata gctcagtcgg
       61 tagagcagcg gccgatatcc gcgggtccag ggttcaagtc cctgttcggg cgccactgca
      121 gatccttagc gaaagctaag gatttttttt aagcttgatg ggggcttagg atccggagct
      181 ccaagcttta gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
      241 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      301 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      361 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
//
