LOCUS       sequence-120581-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CTNNB1
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    164..188
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccacgcgtac ccaacacacg gacacatgca cgaacacgca tgcagagaga tcatacatca
       61 tctatagaga agtacacgta aaagtaagac tcgtcgtctg ccagctacaa ccgagaaggc
      121 gacgtgaaag gaacaagaaa ggaggaggag ggcggccgct tacaccactt tgtacaagaa
      181 agctgggttc aggtcagtat caaaccaggc cagctgattg ctgtcacctg gaggcagccc
      241 atccatgagg tcctgggcat gccccagatc tggcagccca tcaactggat agtcagcacc
      301 agggtggtgg ccacccatct catgttccat catggggtcc atacccaagg catcctggcc
      361 atatccacca gagtgaaaag aacgatagct aggatcatcc tggcgatatc caaggggttc
      421 tccctgggca ccaatatcaa gtccaagatc agcagtctca ttccaagcca ttggctctgt
      481 tctgaagaga gagctggtca gctcaactga aagccgtttc ttgtaatctt gtggcttgtc
      541 ctcagacatt cggaacaaaa cagcagctgc atatgtcgcc acaccttcat tcctagagtg
      601 aagtaactct gtcagaggag ctgtggctcc ctcagcttca atagcttctg cagcttcctt
      661 gtcctgagca agttcacaga ggacccctgc agctactctt tggatgtttt caatgggaga
      721 ataaagcagc tgcacaaaca atggaatggt atttagtcct ctgataacaa ttcggttgtg
      781 aacatcccga gctaggatgt gaagggctcc ggtacaacct tcaactattt cttccatgcg
      841 gaccccctcc acaaattgct gctgtgtccc acccatggac gtacggcgct gggtatcctg
      901 atgtgcacga acaagca
//
