LOCUS       sequence-120583-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 STAT3
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcacatgggg gaggtagcgc actccgaggt caactccatg tcaaaggtga
      241 gggactcaaa ctgccctcct gctgagggtt cagcaccttc accattattt ccaaactgca
      301 tcaatgaatc taaagtgcgg ggggacatcg gcaggtcaat ggtattgctg caggtcgttg
      361 gtgtcacaca gataaacttg gtcttcaggt atggggcagc gctacctggg tcagcttcag
      421 gatgctcctg gctctctggc cgacaatact ttccgaatgc ctcctccttg ggaatgtcag
      481 gatagagata gaccagtgga gacaccagga tattggtagc atccatgatc ttatagccca
      541 tgatgatttc agcaaatgac atgttgttca gctgctgctt tgtgtatggt tccacggact
      601 ggatctgggt cttaccgctg atgtccttct ccacccaagt gaaagtgacg cctccttctt
      661 tgctgctttc actgaatctt agcaggaagg tgcctggagg cttagtgctc aagatggccc
      721 gctcccgctc cttactgata aagcccatga tgtacccttc gttccaaagg gccaggatgt
      781 actttttcac aaggtcaatg atattgtcca gccagaccca gaaggagaag cccttgccag
      841 ccatgttttc tttgcaaaat ttagcccatg tgatctgaca ccctgaataa ttcacaccag
      901 gtcccaagag tttctctgcc agtgtagtca gctgctcgat gctcagtcct cgcttggtgg
//
