LOCUS       sequence-120590-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 STAT6
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaccaactg gggttggccc ttaggtccat gtgggacatt gagatcccag
      241 attgcccata gtgggagggc tgcaggaggg gctgtgcccc caaggaccct ccccccgact
      301 ccccttgccc ctccaggaga agcttagtga ggtcctgttc agtgggaggc agcagaggag
      361 ggaatatgtc ttcaccaatc caagtgccct gaggaaacgc tgtcactggc tggctcaggc
      421 agctgtcttc caccatggtc acatctgagc agagcagggg gtcagggctg gacacagcat
      481 gctcctgagg ctggcacggc agcaggccct gggggtgagg ctggtcaaag ggcaggctca
      541 tctggcccag gctggggggc atctgcaggt gaggctcctg gaaggctgac aacacgttga
      601 ctgattcttc tggggagagg ccttgatacg gggggatgga gtgagagtgt ggtgggtaca
      661 cctggggcac catatctggg ccaagctgca tgctcatgga ggaatcaggg gccattccaa
      721 ggtcataaga aggcaccatg gtaggcatct ggagctctgg ggtaggaagt ggttggtccc
      781 tttccacggt catcttgatg gtagctggga cataacccct gccatcctta cccatctgtt
      841 caggcttgta gtggctccgg aaagcctcat ccttgggctt cttgggata
//
