LOCUS       sequence-120593-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 RAD54B
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..48)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(192..211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(193..211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        243..347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             532..910
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(566..585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acgctcctcg cccttgcttg tgccgtggtg atggtggtga tggtggtgac cagaggtgcc
       61 catggttttc acttacgtgt ttaatgtctt tacagacttt atacattact gtcatggtta
      121 aataaaataa aataaaataa aataaaataa aataaaataa aataaaataa aataaaataa
      181 acttaagatg tccctatagt gagtcgtatt agacgtcagg tggcactttt cggggaaatg
      241 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      301 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      361 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      421 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      481 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      541 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      601 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      661 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      721 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      781 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      841 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      901 caacaacgtt gcgcaactat taactggcga actacttact ctagcttccc ggcaacaatt
      961 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
//
