LOCUS       sequence-120594-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 WWP1
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcattcttgt ccaaatccct ctgtctcttc tattgcaaaa agaagttttt
      241 cctttagttg ttcataactc ttatatggtg gtagatccaa gcgattaaaa catgtatggc
      301 ttcttggtaa ccaagtgtct ttgccaactt tttcaatgca aaacttttga ggcccattac
      361 ttcccatgag ctcagcaaat cctcctagag gtaaacggca ggttccagtg acgaactgca
      421 atagtcgcat tcttacttca ttgtctgtct ctttcacaaa ctgccaaaac caaatgattt
      481 gcttgctgtt tcttgtataa tgtcgataaa cagtatttct ctgccaatct gccaagtcaa
      541 cctcctgcat gccacacaac ataacctcta attctttttc atcgaagtac tgtagccact
      601 gaagaggaac aacttcatta aaaccatcaa ggaaagcttt ggtctgttct tgtactcctc
      661 gagaaaaacg ccattctgtc attaaaccaa tatattcatc tttgttctcc tcagtcacca
      721 gaatattgga acctcccaac ttcaggtcat gtgaagtaac ttttcccaaa atctccatgt
      781 caacagaaaa gtacatttct aagccacatt cttcaatgtt gttatctctt atccagataa
      841 gggagttata aaattcagta tcaatagatt ccaaatcctt aatagtaagt tttttactta
      901 accatacgct tgtagaatgg ta
//
