LOCUS       sequence-120599-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 BMPR2
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcacagacag ttcattccta tatctttaga caccatggtt gtagcagtgc
      241 ctccttctgc aaggtaaaca gcagtgctgg atttggaatg aactgccctg ttactgccat
      301 tattgttgac ttcacagtcc agcgattcag tggagatgac ccaggtggag gggcgccacc
      361 gcttaagaga atagggagtt ttcacacgtt tcttgatctt ttcacctgat cctgatttgc
      421 catcttgtgt tgactcacct atctgtatac tgctgccatc caggacattt gtggctgaaa
      481 gatccagaga attaggcctc tgtgctcttc tgggctttga tggcccagga tctgctgctg
      541 tgcttggaac accctgtgca agaacatctt gttctgaaca tggattgctg ttgttgttat
      601 tggaattagt tcggccacct tctagtggcc gttccctcct gtccacaaga cgatccagaa
      661 caccttcatc atggccagct tgttgctctc gtctcagtaa aggctcatgc tcatcaggac
      721 tggaattaat attcagccgg gtgtcctcac caataaactg attctgcaac atttcttggg
      781 ccctatgtgt cactatgttg gttgtttggc cagatagtac tgtcccattg gcatattggg
      841 ttgtggcagc atgggagtta acactgtggt ttctacctgc cacaccattc atggtgactg
      901 tcaccacatg aggttctgct gcattgattg tattcatctt ggcaactcc
//
