LOCUS       sequence-120601-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 POLD1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaccaggct caggtccagg gggtccgaag cgccgcagga gctgctcctg
      241 gtcttccagg tccttccgca ccttcttgcg catgtagaag atggggcagt cccggctggt
      301 gcagatgacg tcctcgtgca ggctgccctg gcagcgctgg cactgcgtcc agaggcgcga
      361 gaagcgctcc tccagggcat tcagatggga tacctccttc tgatacagct cagactcccg
      421 gggctggcag aactcacaca cggctccctg gtggctgagc actgtgcggc agccaatgca
      481 gcagttgcgg cgtttggcga aggccaggag gccgcccacc ttgcccgtga gcaccgtctt
      541 gcagcgcgtg tggtcccccc gcagtagcac agcctcggca cggccctcgc ccaggatggg
      601 ctcgaagatg cgcaggaggg gcttggccag ctgctgctcc aggtagtact gcgtgtcaat
      661 gggcaggctg tgctccagca cgaacagcgg gtcctccgac ttcatgtagg cggccacacc
      721 cttggcggca ctgatgatca cgtaggggac gcggtcgccc aggctgggcg cactcccggg
      781 gtcccgcttc ctcatcctct cggccagctc cacgtgggcc tgcttgccgg catagtcgga
      841 ggccgcgcgg gtcagctcct tggtgatgac cagctgggag atatcgatgc ggttgcacag
//
