LOCUS       sequence-120602-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 POLD1
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..48)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(191..210)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(192..210)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        242..346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             531..909
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(565..584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaagctctcg cccttgcttg tgccgtggtg atggtggtga tggtggtgac cagaggtgcc
       61 catggtttca cttacgtgtt taatgtcttt acagacttta tacattactg tcatggttaa
      121 ataaaataaa ataaaataaa ataaaataaa ataaaataaa ataaaataaa ataaaataaa
      181 cttaagatgt ccctatagtg agtcgtatta gacgtcaggt ggcacttttc ggggaaatgt
      241 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      301 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      361 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      421 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      481 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      541 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      601 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      661 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      721 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      781 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      841 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
      901 aacaacgttg cgcaaactat
//
