LOCUS       sequence-120604-       11739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax MSP1 ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MSP1-bio
FEATURES             Location/Qualifiers
     source          1..11739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7119..7174
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7173..7192)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9410..9428)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9496..9600
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9601..9669,9670..10461)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9819..9838)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10632..11220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11121..11140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11374..11391
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11508..11529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11544..11549,11550..11567,11568..11574)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11582..11598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11587..11609
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11606..11622
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11606..11622
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcagaaa ccgagagcta caagcagctg gtggccaagc
     1381 tggataagct ggaagccctg gtggtggacg gctacgagct gttccacaag aagaagctgg
     1441 gcgagaacga catcaaggtg gaaacaaacg ccgctgccaa caacaacaat aacaaccagg
     1501 tgtccgtgct gaccagcaag atccggaact tcctgagcaa gttcctggaa ctgcagatcc
     1561 ccggccacac cgacctgctg cacctgatcc gggaactggc cgtggaaccc aacggcatca
     1621 agtacctggt ggaaagctac gaggaattca accagctgat gcacgtgatc aacttccact
     1681 acgacctgct gagagccaag ctgcacgaca tgtgcgccca cgactactgc aagatccctg
     1741 agcacctgaa gatcagcgac aaagaactgg acatgctgaa gaaagtggtg ctgggctacc
     1801 ggaagcccct ggacaacatc aaggacgaca tcggcaagct ggaaaccttc atcaccaaga
     1861 acaagatcac catcaagaat atcgccgacc tgatcattgc cgagaacaag aagcggagcg
     1921 gccaccctac caccaccacc aatggcgccg gaacccagcc cgccaatggc gctattgccg
     1981 ccgctagcag cgagacaacc cagatcagcg gcagcagcaa cagcggcagc tccagcaccg
     2041 gctccagcaa ctctggcagc agcagcacag gcagcagcgg aaccggctct accggcacag
     2101 gacagtctcc ccctgccgcc gctgacgcca gcagcaccaa tgccaactac gaggccaaga
     2161 agatcatcta ccaggccgtg tacaacacca tcttctacac caaccagctg caagaggccc
     2221 agaaactgat cgccgtgctg gaaaagcgcg tgaaggtgct gaaagagcac aaggatatca
     2281 aggtgctgct ggaacaggtg gccaaagaga aagagaagct gcccagcgac taccccaaca
     2341 ccgccaacct ggccaacgtg cacaaagagg ccgagagcaa gattgccgag ctggaaaaaa
     2401 agatcgaggc cattgccaag accgtgaact tcgacctgga cggcctgttc accgacgccg
     2461 aggaactgga atattacctg cgcgagaagg ccaagatggc cggcaccctg atcatccccg
     2521 agagcaccaa gagcgccggc acacccggca agaccgtgcc caccctgaaa gagacatacc
     2581 cccacggcat cagctacgcc ctggccgaga acagcatcta cgagctgatc gagaagatcg
     2641 gcagcgacga gacattcggc gacctgcaga accccgacga cggcaagcag cccaagaagg
     2701 gcatcctgat caacgaggcc aaacggaaag agctgctcga gaaaatcatg aacaagatta
     2761 agatcgaaga ggacaagctg cccaacctga agaaagagta cgaagagaag tacaaggtgt
     2821 acgaagccaa agtgaacgag ttcaagcccg ccttcaacca cttctacgag gcccggctgg
     2881 acaacacact ggtggaaaac aagttcgacg acttcaagaa gaaacgcgag gcctacatgg
     2941 aagagaagaa aaagctggaa tcctgcagct acgagcagaa cagcaacctg atcaacaagc
     3001 tgaagaagca gctgacctac ctcgaggact acgtgctgcg gaaggacatt gccgacgacg
     3061 agatcaagca cttcagcttc atggaatgga agctgaagtc cgagatctac gacctggccc
     3121 aagagatccg gaagaacgag aacaagctga ccatcgagaa caagtttgac ttcagcggcg
     3181 tggtggaact ccaggtgcag aaagtgctga tcatcaagaa gattgaggcc ctgaagaacg
     3241 tgcagaacct gctgaagaac gctaaagtga aggacgacct gtacatcccc aaggtgtaca
     3301 agaccagcga gaagcccgag ccctactacc tgatggtgct gaagagagag atcgacaagc
     3361 tcaaggactt catccccaag atcgagagca tgatcgccac cgagaagaac aagcccaccg
     3421 tggccgctgc cgacatcgtg gccaagggcc agagcctgag aggcgccagc gagacaggca
     3481 ccaccggcaa caccgtgaac gcccagacag ccgtggtgca gccccagcac caggtggtca
     3541 atgccgtgac cgtgcagccc ggcacaacag gacatcaggc ccagggcggc gaggccgaga
     3601 cacagacaaa tagcgtgcag gccgcccagg tccagcagac accagctggc gctggcggcc
     3661 aggtggccag cacacagaca atctctcagg cccctgcccc cacccaggcc tctccagaac
     3721 cagctcctgc cgcccctcct agcacaccag ctgctgctgt ggccccagcc cctaccatgt
     3781 ctaagctgga atatctggaa aagctgctgg acttcctgaa gtccgcctac gcctgccaca
     3841 agcacatctt cgtgaccaac agcgccatga agaaagaact gctggaccag tacaagctga
     3901 acgccgatga gcagaacaaa atcaatgagg ccaagtgcga cgagctggac ctgctgttca
     3961 atgtgcagaa caacctgccc gccatgtact ccatctacga cagcatgagc aacgagctgc
     4021 agaatctgta catcgagctg taccagaaag agatggtcta caacatctac aagaacaagg
     4081 acaccgataa gaagatcaag gccttcctgg aaaccctgaa gtccaaggcc gctgcccctg
     4141 cccagagcgc cgccaaacct tctggccagg ccggcaccac ccctgtgacc accacagccc
     4201 cagtgaccac aaccaccgtg acccccagcc cccagaccag cgtcgtgacc agcacccctc
     4261 ctacccctca ggccgaagag aacagaagag tgggcggcaa cagcgaggaa aagcccgagg
     4321 ccgacaccgc ccaggtggaa aagttctacg agaagcacct gagccagatc gataagtaca
     4381 acgactactt ccagaagttc ctcgagagcc agaaggacga gatcaccaag atggacgaga
     4441 caaagtggaa ggccctgggc gccgagatcg aggaactgaa gaagaaactg caggtttccc
     4501 tggaccacta cggcaagtat aagctgaagc tggaacggct gctcaagaag aagaacaaaa
     4561 ttagcaacag caaggaccag atcaagaagc tgaccagcct gaaaaacaaa ctggaacggc
     4621 gccagaatct gctgaacaac cccaccagcg tgctgaagaa ttacgccgcc ttcttcaaca
     4681 aaaagcgcga gacagagaag aaagaggtcg agaacaccct caagaacacc gagatcctgc
     4741 tgaagtacta caaggccaga gccaagtact acatcggcga gcccttcccc ctgaaaaccc
     4801 tgtccgaaga gagcatgcag aaagaggaca actacctgaa cctcgagaag ttccgggtgc
     4861 tgagccggct ggaaggccgg ctgggcaaga acatcgaact ggaaaaagag aacattgcct
     4921 acctgagcag cggcctgcac cacgtgctga ccgagctgaa agagatcatc aaaaacaaga
     4981 agtacagcgg caacgaccac acgaaaaaca ttgccgccgt gaaagaggcc ctgcaggcct
     5041 accaagagct gatccccaaa gtgaccaccc aagagggcgc cagcaccacc gccgccacac
     5101 tgcctgtgac agtgccttct gccgtgcctg gcggactgcc aggtgctggt gttcctggcg
     5161 ctgccgccgg actgacacct cctccaccag ccggatctgt gcctgccaca ggaccaggcg
     5221 ccgctgccgg ctccacagag gaaaatgtgg ccgccaaggc ccaggactac gccgaggatt
     5281 acgacaaagt gatcgccctg cccctgttcg gcaacaacga cgacgacggc gaagaggacc
     5341 aagtgaccac aggcgaagcc gagagcgagg cccctgagat cctggtgcct gccggcatca
     5401 gcgactacga cgtggtgtac ctgaagcctc tggccggcat gtacaagaca atcaagaaac
     5461 agctcgagaa ccacgtgaac gccttcaaca ccaacattgc cgatatgctg gacagccggc
     5521 tgaagaagag aaactacttc ctggaagtgc tgaacagcga cctgaacccc ttcaagtaca
     5581 gcagcagcgg cgagtacatc atcaaggacc cctacaaact gctggatctc gagaaaaaaa
     5641 agaagctgat cggcagctac aagtacatcg gagccagcat cgacatggac ctggccaccg
     5701 ccaacgacgg cgtgacctac tacaacaaga tgggcgagct gtataagacc cacctggatg
     5761 gcgtgaaaac cgagattaag aaggtcgagg acgatatcaa gaagcaggac gaggaactca
     5821 aaaagctggg caacgtgaac agccaggaca gcaagaagaa cgagtttatc gccaagaagg
     5881 ccgaactcga gaagtacctg cccttcctga acagcctgca gaaagaatac gagagcctgg
     5941 tgtccaaagt caacacctac accgacaacc tcaagaaagt gatcaacaac tgccagctcg
     6001 aaaagaaaga agccgagatc accgttaaga agctgcagga ctataacaaa atggacgaga
     6061 agctggaaga gtacaagaag tctgagaaaa agaacgaagt gaagtccagc gggctgctcg
     6121 aaaagctgat gaagtctaag ctgatcaaag agaacgaggc taaagagatc ctgagccagc
     6181 tgctgaatgt gcagacacag ctgctgacca tgagcagcga gcacacctgt atcgacacca
     6241 acgtgcccga caacgccgcc tgctaccggt atctggacgg caccgaggaa tggcggtgcc
     6301 tgctgacctt caaagaagag ggcgggaagt gcgtgcccgc cagcaacgtg gcctgcaagg
     6361 acaacaatgg cggctgcgcc cctgaggccg agtgcaaaat gaccgacagc aacaagatcg
     6421 tgtgcaagtg caccaaagag ggcagcgagc ctggcgcgcc gtcgacctcc atcacggcct
     6481 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     6541 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     6601 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     6661 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     6721 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     6781 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     6841 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     6901 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     6961 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggat
     7021 cactgcatca tattctggat gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac
     7081 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     7141 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     7201 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     7261 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     7321 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     7381 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     7441 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     7501 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     7561 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     7621 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     7681 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     7741 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     7801 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     7861 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     7921 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     7981 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     8041 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     8101 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     8161 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     8221 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     8281 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     8341 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     8401 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     8461 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     8521 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     8581 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     8641 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     8701 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     8761 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     8821 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     8881 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     8941 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     9001 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     9061 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     9121 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     9181 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     9241 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     9301 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     9361 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     9421 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     9481 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9541 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9601 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9661 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9721 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9781 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9841 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9901 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9961 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    10021 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    10081 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    10141 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    10201 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    10261 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    10321 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    10381 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    10441 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    10501 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10741 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    11041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    11101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    11161 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    11221 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    11281 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    11341 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    11401 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    11461 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    11521 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    11581 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
    11641 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
    11701 tgttgccatt gctgcaggcg cagaactggt aggtatgga
//
