LOCUS       sequence-120606-        9108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax MSP3.1 (MSP3gamma) ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MSP3.1-bio
FEATURES             Location/Qualifiers
     source          1..9108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4488..4543
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4542..4561)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6779..6797)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6865..6969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6970..7038,7039..7830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7188..7207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8001..8589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8490..8509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8743..8760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8877..8898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8913..8918,8919..8936,8937..8943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8951..8967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8956..8978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8975..8991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8975..8991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaaca acgtggtgtc caacgagaac gtgaacctga
     1381 agaaccccaa cctgcggaac ggctgggccg gcaaaaatgt ggccctgcag gatgagcaga
     1441 gcggcctggt ggctgaggat ggcgaggaag tgaacaccaa agaggaccag aacgagctgc
     1501 tgcacaagag cgaggaaacc gacttccaga cgcagggcca ggaagtgaaa gagatcggcg
     1561 gcagcgagaa cgagaagaag cagaagcaga tcaagaaggc caacgaggcc aaagagaagg
     1621 ccctgaaaga gcagaaagag gccgaggacg aagtgaagaa gatcgaagag gccctggaca
     1681 aagtgaaaaa ggccaaggcc gaagccgagg aagaagccga gatcaaaaag gtggtggaaa
     1741 aggctaaggg cgtggccgac aaagccaaag aaaccgccat gaacaaggcc aaaaagagca
     1801 aggacgaggc cgaaaagatc gccggcgtga acacaaacag catggcctac ctgtacgccg
     1861 gcaacgccgt gaccgccgag attgaagcca aagccgagaa agagaaagcc aaaaaggccg
     1921 ccgaaatcgc caagcacgcc gtggatgcct acgagctgaa gaaagaagct gagaaagccc
     1981 aggaagaggc tgaggccgcc aagaccgaga tcgagaagct gtccaaggtg tacaaagagg
     2041 gcaacgtggc cgaggccgtg cagtctacag ccgaggctga gaagtctgcc tctggcgcct
     2101 ccgccaaagt gaaagaagcc gcccacaatg tggccaagaa actgatggac gccgtgcaga
     2161 aactggaaaa ggtgtccacc gagctgccca aggacaacga gcaggccgcc accatcgaca
     2221 acgtgaacga ggtcgtgaca gaggccgtga aagaaaaaga aaaggccatg atcagcgccg
     2281 aggtggccaa agcagaggcc gctaatgccg aagcccagct ggccaagatc gaggccgaga
     2341 gagccaaata cgaagccaac aagattgccg aagagtacac cgacaatgtg aagggcgagg
     2401 ccaagaaggc tgaggaaaaa gccaacgaag ccagcagcaa ggccacagag gccagcaaca
     2461 atgccaaggg cgccagcggc gaggaaaagc agacaaatcc ccaggctgcc aacgtgaagg
     2521 caaaggccgg cgaagccatc aaagccgcca aagaggctaa gaaagctaag accgaggcct
     2581 atatcgccct gtgcgtgacc aagaccctgg tggcaaaaga gaacgcaaag aaagccgagc
     2641 aggaagccaa gaacgccaag gataaggcca ccaaggctgc taaagaggca gaagaagcta
     2701 aaaagcaggc cgagaaggca gagaagatca ccgagacagt gaagaatgag gccaaaaccg
     2761 ccaccgatga ggaagctaag gccagcaccg gcaagaagga cgctgagatc aacgccggct
     2821 acgtggacga agaggtgtac gccgtgaaca tcgagttcga gatcgccaaa gaagcagcca
     2881 agactgccgc ccagcacaaa gccctggaaa tcctggacaa ggcagaaaaa aacgccgaga
     2941 tcgctgccga gaacgccgct gccaaagctc aggaagctac taagaaggcc gaaactgcca
     3001 agacaaaggc cactgaagcc gaaaccgccg caaagaaggc ccaggacgcc agcgagaagg
     3061 ctaaagccat tgccgccgac gtgctggccc agaaggcctc tacagaagcc cagagcctga
     3121 aacaggaagc agagaagctg gccgaaaaca tcaagaaatc caacgtggca gatgaggaaa
     3181 aggcaaaagc cgacgaggca gccaaagctg ctaaggacgc cgccgaccag gcctctgcca
     3241 gcgccaagaa agcaaacgac gccaagattg ctgccaccaa cgcccaggtg gtcgtgaccc
     3301 tgcagaccaa aaaagctgag agcgccaagg ctgaagatgc cgctaaagaa gccatgaagg
     3361 cccgggacaa ggccgccttt gagctgctga agattaagaa acaggatgtg ctggaacagg
     3421 tggacgtgtc ccccagcggc tccgacaacc tgaatgatgt ggatgaacag gtggccctgg
     3481 aagtgggcga gcagcagaat gaagcagagg acgccgagcc ccaggaagct gaagagggcg
     3541 acgaggaaga tgaagaggac accgaagagg aagagatcca ggacgagagc gaccacaccg
     3601 aggaaagctc tgccaagcag gctgcccagc aggaaaaaca gcagggcgaa aaggtgctga
     3661 atgacgaaga agcccacaat ctgctggctc agcagcacaa caaggataac aacgccgcca
     3721 ctgaggatgc caagctgttc cagaccatca tcaaggactt cgacgacgac gatgacttca
     3781 agaacctgca gaaagacgtg aacgccctgt tcaagatcaa aggcgcgccg tcgacctcca
     3841 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     3901 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     3961 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     4021 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     4081 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     4141 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     4201 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     4261 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     4321 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     4381 attccggatc actgcatcat attctggatg cacagaaaat ggtgtggaat catcgttaag
     4441 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     4501 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     4561 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     4621 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     4681 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     4741 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     4801 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     4861 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     4921 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     4981 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     5041 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     5101 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     5161 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     5221 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     5281 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     5341 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     5401 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     5461 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     5521 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     5581 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     5641 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     5701 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     5761 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     5821 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     5881 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     5941 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     6001 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     6061 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     6121 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     6181 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     6241 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     6301 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     6361 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     6421 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     6481 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     6541 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     6601 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     6661 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     6721 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     6781 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7201 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7501 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8701 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8761 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8821 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8881 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8941 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9001 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     9061 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatgga
//
