LOCUS       sequence-120608-        9624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax MSP3.3 (MSP3beta) ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MSP3.3-bio
FEATURES             Location/Qualifiers
     source          1..9624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5004..5059
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5058..5077)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7295..7313)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7381..7485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7486..7554,7555..8346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7704..7723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8517..9105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9006..9025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9259..9276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9393..9414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9429..9434,9435..9452,9453..9459)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9467..9483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9472..9494
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9491..9507
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9491..9507
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgata atgtggctac cagaggcgag atcgtgaacc
     1381 tgaagaaccc caacctgcgg aacggctggt ccatgaagaa tctggccgcc cagaacgaag
     1441 agaacatcgt gcacagcgac ggcagcgacg acgtgaccga caaagaagag gacggcgagg
     1501 tgctggaagg ccagaagggc agccctaaga agtccgccga gcagaaggtg cacgcccagg
     1561 aagaagtgaa caaagagagc ctgaagtcca aggcccagaa tgccaaggcc gaggccgaga
     1621 aggccgccaa agctgccgag agcgccaaag agaataccct ggacgccctg gaaaaagtga
     1681 atgtgcccac cgagctgaac aacgagaaga acttcgccga gtccgccgcc acagaggcca
     1741 agaagcagga aaagatcagc accgaggccg ccgaggaagt gaaagaaatc gaggtggacg
     1801 gccagctgga aaagctgaag aatgaggaag agaaaaccgc caagaaggcc cggaaacagg
     1861 aaatcaagac cgagattgcc gagcaggctg ccaaggctca ggcagccaag acagaggccg
     1921 aaacagccca gaaggatgcc accaccgcca aggatgaggc catcaaagag acaggcaagc
     1981 ccaagagcca gaacaccgct aaggccgtga ccatggccac cgaagaggaa aaaaagacca
     2041 aggacgaagc ccagaccgcc agcgagaaag ccggcaagac agccgaagag gctcagaaag
     2101 aagtgggcaa agaaaccgcc gacgacgaca aagaggtgtc acagctggaa gaagaaatca
     2161 aagagctgga acggatcctg aagattgtga aggacctggc cagcgaggcc agcagcgcca
     2221 gcgataatgc caagaaagcc aagctgaaaa cccagatcgc cgccgaagtc gtgaaagccg
     2281 aaaaggccag gatcgaggct gaggaagccg agaaagaagc cggcgaagcc aagaccaaaa
     2341 ccgaggccac agagaaagag gtgctgaaga tcagcgacga gagcaaggcc gctaaagtga
     2401 agaaagccgt ggaaaaagcc aaagaagctg agaagcaggc caagtctgag gctgaaaagg
     2461 ctaagggcat ggccgacgat gccggcggaa agggcaccac caacctggaa gatgtgctga
     2521 ccaagctgag cgaagtgctg acctccgtga agtccctggc ctccaatgcc gaggtggcca
     2581 gcaagaacgc caaaaaagag atgaccaagg cacagattgc cgctgaagtg gccaaggctg
     2641 agaaagcaaa gatcgaggca gagaacgcta aactgctggc cgacaccgcc agcaaggcag
     2701 ccgagaatat cgccaagtcc agcaaagccg ccaagatcgc caacaacgtg gccaccattg
     2761 ccgccgagaa aagcaaggtg gcaacagagg ctgccgacga ggccgcaaaa gccctggacg
     2821 agacagagaa ccccgagtct aagatcgccg aagtgacaga gaaggccaca aaggctgtga
     2881 acgccgcaga ggaagctaag aaagagaagg ctaaagccga agtggccgtg gaagtggccc
     2941 acgcagaagt ggctaaagag aaagcacagg aagcaaaaga agcagccaaa caggtggccg
     3001 acaagagcaa actggaaaag gcaatccagg ccgctgacaa ggcctccgag aaagctaacg
     3061 aggcctccaa gctggccgaa gaagccctga gcaatctgga atctctggaa aaagaaaccg
     3121 gggagatcgt ggaaaaagtg aacgccatcg agcagaaagt gcagaccgcc aagaacgccg
     3181 ccatcgaggc ccacaaagag aaaacaaagg ccgagatcgc tgtggaagtg gctaaggccg
     3241 aggaagccaa aaaagaggcc gacaacgcca aggtggccgc agagaaggca aaagagacag
     3301 ccgagaagat tgctaagacc tccaagagca cagagaagat cacagaggaa gtgcgcaagg
     3361 ccaccgagtt cgccaaaaca gccggcgacg agacaaccct ggccgccaca aaagccgaat
     3421 ccgagatccc cagcgaggaa aagaaccaga aagaactgct ggacagcatc aagcagaagg
     3481 cagagagcgc cttccaggcc agccaggaag ccatcaaggc caagaccgaa gcagagaatt
     3541 tcctggaaat cgctaaagaa gtgcccaaag ccgaggctgc caaagaagaa gcccagaaag
     3601 ccgctacagc cgcagaagag gcaaagacag aagtgctgaa aatcgctgag gaagtgaaca
     3661 aggccgacgc cagcgagagc gagaagaaga agatcgagac tgccgccaat gaggccgctg
     3721 gcgaggcaga aaaagctgcc acctttgcca aagaggcagc tgacgccgct aaggacacca
     3781 acaaagccgt gacactggcc gtggccaaag aaaaggtgga aaaggccctg aaggctgcta
     3841 aagaggccaa aaaggccaac gaaaaagcct cctacgccct gatccggacc aagaaacagt
     3901 atgccctgga accactggaa atcacctccg aggccggcta caacattgcc gaaaaagagg
     3961 aacaagtgaa agaggaaatc gaggaacagg acgacaaggc cagcgaggaa gaggaagagg
     4021 acacccagca gatcgaccag acacagatcg atgaggtgga catcagcgtg gacaacgagg
     4081 aagaagagga aggggccgct gaggaacaga tcgagggcga gaaggacacc cccaccaaag
     4141 aagccaaaga ggaacagacc tccggcgaga agatcctgga tgacaaagaa gcccacaaga
     4201 ccctggccga gaagttcaag gacagcaaca ccgccaaaac cggcggagtg gaattcctgg
     4261 aaaccctgat ctccgacgtg ggcgaggaca cactgaaaaa cctgcagcag gacctgcacc
     4321 agtacttcaa gggcaaaggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     4381 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     4441 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     4501 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     4561 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     4621 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     4681 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     4741 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     4801 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     4861 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggatcactg catcatattc
     4921 tggatgcaca gaaaatggtg tggaatcatc gttaagtcta gacacgtgat taattaagga
     4981 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     5041 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     5101 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     5161 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     5221 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     5281 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     5341 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     5401 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     5461 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     5521 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     5581 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     5641 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     5701 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     5761 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     5821 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     5881 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     5941 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     6001 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     6061 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     6121 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     6181 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     6241 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     6301 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     6361 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     6421 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     6481 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     6541 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     6601 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     6661 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     6721 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     6781 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     6841 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     6901 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     6961 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     7021 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     7081 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     7141 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     7201 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     7261 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     7321 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7381 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7441 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7501 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7561 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7621 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7681 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     7741 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7801 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7861 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7921 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7981 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     8041 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8101 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8161 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8221 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8641 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     9301 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9361 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9421 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9481 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     9541 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     9601 aggcgcagaa ctggtaggta tgga
//
