LOCUS       sequence-12061-e       12944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Tet-based inducible shRNA or cDNA expression, 
            constitutive Zeocin resistance gene coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Zeo
FEATURES             Location/Qualifiers
     source          1..12944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2738
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2763..2768,2769..2786,2787..2793)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2847..3506
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2913..2932)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3848..4153
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4032..4051
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4194..4318)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4368..5579
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5416..5435
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5638..6255,6256..6261,6262..6297,6298..6300,6301..6336,
                     6337..6339,6340..6375,6376..6384)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6422..6996
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6589..6606)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        7018..7065
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7084..7458
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7632..8220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7685..7705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8103..8114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8223..8239)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8224..8240)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8749..8929
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8955..8972)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8961..9185
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9231..9659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9318..9337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9528..9549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9668..9688)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9673..10002
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9853..9988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9915..9934
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10050..10097
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10116..10490
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10620..10741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10657..10676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10711..10730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10790..10806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10790..10806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10803..10825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10814..10830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10838..10844,10845..10862,10863..10868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10883..10904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11021..11038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11192..11780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11272..11291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11951..12742,12743..12811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12574..12593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12812..12916)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agctgaacga
     2641 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     2701 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
     2761 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccggcga
     2821 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     2881 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     2941 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     3001 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     3061 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     3121 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     3181 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     3241 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     3301 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     3361 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     3421 atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     3481 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac
     3541 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     3601 gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga
     3661 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     3721 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     3781 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac
     3841 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     3901 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     3961 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     4021 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     4081 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     4141 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
     4201 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     4261 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
     4321 ataacatctc gagccataaa gatggttaat taacccaccc aagatctggc ctccgcgccg
     4381 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4441 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4501 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4561 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4621 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4681 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4741 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4801 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4861 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4921 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4981 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     5041 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     5101 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     5161 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5221 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5281 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5341 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5401 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5461 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5521 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5581 aagcttggta ccgagctcgg atctccaccc cgtaccggtc ctgcagtcga attcaccatg
     5641 tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
     5701 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
     5761 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
     5821 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
     5881 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
     5941 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
     6001 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
     6061 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
     6121 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
     6181 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
     6241 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
     6301 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
     6361 gaccttgaca tgctccccgg gtaactaagt aaggatccgc ggccgcacta gaggaattcc
     6421 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     6481 gtgtgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     6541 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     6601 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     6661 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     6721 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     6781 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtagtcaaca
     6841 aggggctgaa ggatgcccag aaggtacccc attgtatggg aatctgatct ggggcctcgg
     6901 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6961 ggggacgtgg ttttcctttg aaaaacacga tgataagctt accggtacgc gtgatgtgtt
     7021 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     7081 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     7141 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     7201 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     7261 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     7321 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     7381 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     7441 gccgaggagc aggactgaca catctgtaca agtaaagcgg ccgcgactct agatcataat
     7501 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7561 gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa ttcgatatca
     7621 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7681 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7741 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7801 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7861 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7921 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7981 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     8041 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8101 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8161 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8221 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     8281 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     8341 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8401 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     8461 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     8521 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     8581 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     8641 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     8701 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     8761 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8821 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8881 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     8941 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     9001 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     9061 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     9121 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     9181 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     9241 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9301 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9361 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9421 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9481 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9541 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9601 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     9661 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     9721 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9781 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     9841 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9901 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     9961 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
    10021 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
    10081 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
    10141 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
    10201 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
    10261 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
    10321 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
    10381 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
    10441 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
    10501 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    10561 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
    10621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10741 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
    10801 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10861 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    10921 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10981 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    11041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    11101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    11161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    11221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    11281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    11341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    11401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    11641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    12001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    12061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    12121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    12181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    12241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    12301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    12361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    12421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    12481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    12541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
