LOCUS       sequence-120610-        9906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax MSP3.4 (PVX_097685) ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MSP3.4-bio
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5286..5341
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5340..5359)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7577..7595)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7663..7767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7768..7836,7837..8628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7986..8005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8799..9387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9288..9307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9541..9558
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9675..9696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9711..9716,9717..9734,9735..9741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9749..9765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9754..9776
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9773..9789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9773..9789
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaatg tggtgtccaa cgagatcgtg aacctgaaga
     1381 accccaacct gcggaacggc tgggccggca aggatgtgac actgcaggac gagcagaacg
     1441 gcctgaacgc cgaggatggc gacgagacag atcccaacgg cgcccacaat gagctgctgc
     1501 agaagtccgg aaaggccgag ctgcagatgc aggcccagga cagcgacagc aaagaggaca
     1561 tcaagaccaa gatcgacgag ctcgtgaaca aggccgaggc cgccaacacc aaggccgata
     1621 cagccgtgac agaggccgag aagctgctga aaaccgccac cgaggccgaa aagcagatca
     1681 aggtggacga ggaaaaagag acaaccgccg ccacaaaggc caaagagcag gctgagaagg
     1741 cctttgccga ggccaagatc aagaaagagg aagccaagac aaaaaaagag gacgctaagg
     1801 ccaaggctaa ggccgacgac gccaagaaga agtccgagga aaccgacgaa gccgagctga
     1861 aggccaagct ggaagaggtg gaaaagctcg tgggccaggc cgaacaggcc gcctctgatg
     1921 ccgagaatga gcagaaaaag gccgaatggg ccgccaagat tgccgaggaa ctgaagaagg
     1981 cctacgaggc caaaaaaaag gcccgcgaga gcaaggtggc cgccgagaac gccaaaaacg
     2041 atgccacatc tgccgccagc ggcagcaagg tgcagaaagt gaccgaggcc atcaagatcg
     2101 ccgacgatga gatgcagacc gccgctaaga atgccgccga agccgaaggc gccgagagaa
     2161 aagctgagca ggccgctagc gaggccatta acgccatcga gcagaaggtg gacgcccaga
     2221 gcaaagtgaa cgtggtgcag aacgaggccg acgctgcctc taaagccgcc accgctgcca
     2281 agaacgccca gaccaaggct gagatcgccg tgttcgtcgt gaaaaccgag gtgtccaaag
     2341 aagaggccgt gaagtccgcc aacgccgccg aggaagctaa aacaaaggct gaggaagtgg
     2401 ccaccaagag cgaggaaatc aagatcacca acgtggccaa gtccgagaaa gtgaaaagcg
     2461 ccaagtctga agccgtgaat caggccgctg ccgccgacaa ggcaaagaaa gaagctgagg
     2521 ctgccgccaa ctctgccgat aaggcccaga caagcaccaa gggcatcgac aacgaggcca
     2581 ccctgagcac cgccaaaaaa gaagccgaaa cagccgccag caacgccggc aaagcccaga
     2641 cagatgcctc taccgccctg gccaaagcca agaaggctca gatcgagaca gagatcgctg
     2701 ccgaggtcgt gaaggcagaa aaggctaaga aggacgccag cagcgacgcc gatgccgctg
     2761 tggaagccag aaagaaagcc gagagcgagg cagagaaagc caagtccaat gaggtggccc
     2821 aggaagccgc ccagaaggct aaagaacagg aagccgaggc cctgaaagcc aagggcaatg
     2881 cccagaccgc cagcgaccag gccgaaaaag aagcagaggg cgacggcacc gaggacaagc
     2941 tggctaaagt gaaagagaaa gaaaaggacg cctccaatgc cgccacagcc gctaaaaacg
     3001 ccagaaccac agccgagatt gccgcagagg tggccaaggc ccaggctgct aagacagagg
     3061 cagaggccgc taaagtggcc gctaacgcag ccaaggacga ggctcagaaa gtggctgacg
     3121 agtccaagag cgctgaggca cagaaggccg cagagaaggc tagcgaagcc gctacaacag
     3181 ccggcgagaa agccgctgaa gctgccaagg tggcagatga cgccaccagc gtgtccaaca
     3241 ccgccaccct gtccaccatg caggaaaagg ccaacaaagc cgtggaacac gccaaagaag
     3301 caaaaaaggc acagaccaaa gcagagctgg ctgccgaaga agccaaggca gaagcagcta
     3361 agaaagaggc ccagaaagct caggaagctg ccgacgccgc taaaaaagag gccgaggaaa
     3421 tcgccaagaa agaaaaagtg gatgccaagg ctgtggccga agccacctcc gccctggata
     3481 ccgcaaagac taaggcaaaa gaggcaaagg acaaggccgg cgacgtgtca aaagaagtgg
     3541 aaaaagctga aaaagaggtg gaaaaagtcg tgggggacga cgcccagaaa agcgagatca
     3601 ccacagccgt gtccggcatc aagggcaaga cctctgaggc cgtgcaggcc gcaaaggatg
     3661 caaaaaaagc caaagacctg gccgagatcg ccctgaatgt cgtgaaagct gaagtggcta
     3721 acgacaaggc taaaaaggct gtgcccgctg ccggcgtggc caaagaggat gccaaaaagg
     3781 ctgccgctag cgccgagcag cccaagaaag aggctgagag cgccgaggaa ggcgccgaaa
     3841 tcgccaaaga gaacgagatc gaggccggcc agaaagccaa agaagccgag gataacgccg
     3901 ccgtggtgca ggccaaactg ctgatcgccg agcaggaact gaaaaacgcc aaggccgaga
     3961 acgacgccga gaagttccag agcgccaagg caaatgccct ggccgctgct gaggaagctg
     4021 tgaagaaagg ccaggccgca gaagccgctg ccaacgctgc tacagacaaa gccaaaaaag
     4081 ctgctgaggc cgccgaaaag gctaaaaaag ccgctgccga gtccgccctg aagaaaaaac
     4141 tgaaggtgct ggaaatcgtg aagaagtaca gcaagcgggg ctacaacgtg gtggacaacg
     4201 atgaacaggt gctgaacgaa gtggatgagc aggcttccga agctaaagaa gaagaggacg
     4261 aggacgaagc agacgacagc gccagctccg acgtggaaat cgaggacgac gaggaagagg
     4321 aagaagaaga ggacgaagaa gtgaaagagg aagagggcca ggcccagagc agcgcccatc
     4381 agaatagtgt ggctgagctg gaaaaccaga agaaagagag ccaggaaaag tccgacgagc
     4441 cccccagcga caacaacgct caggccctgc tgagcgacaa ctacaagaac ttcgccgact
     4501 tcaagaagaa agctgaggac ctgaccaaga acatcatcaa caccatcgac ggcgacgctg
     4561 gcgtgatcga caccctgaag gacttcgcca acgacgtgaa ccagttcatc ctgaacatgg
     4621 gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg gagttctcct
     4681 tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag gcagagaagg
     4741 ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga
     4801 agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc cttcagatac
     4861 cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg gacagaggga
     4921 tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac agcaacactt
     4981 tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg aagcaggaga
     5041 atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc cctgaagcag
     5101 gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc aagatccagg
     5161 ttttatccaa agggttgaat tccggatcac tgcatcatat tctggatgca cagaaaatgg
     5221 tgtggaatca tcgttaagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     5281 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     5341 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     5401 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     5461 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     5521 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     5581 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     5641 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     5701 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     5761 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     5821 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     5881 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     5941 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     6001 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     6061 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     6121 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     6181 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     6241 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     6301 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     6361 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     6421 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     6481 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     6541 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     6601 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     6661 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     6721 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     6781 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     6841 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     6901 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     6961 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     7021 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     7081 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     7141 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     7201 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     7261 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     7321 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     7381 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     7441 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     7501 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     7561 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7621 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7681 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7741 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7801 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7861 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7921 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7981 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8041 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8101 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8161 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8221 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8281 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     8341 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8401 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8461 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8521 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8581 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8641 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8941 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9421 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9481 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9541 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9601 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     9661 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     9721 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     9781 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     9841 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     9901 tatgga
//
