LOCUS       sequence-120612-        9120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax MSP3.10 (MSP3alpha) ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MSP3.10-bio
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4500..4555
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4554..4573)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6791..6809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6877..6981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6982..7050,7051..7842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7200..7219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8013..8601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8502..8521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8755..8772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8889..8910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8925..8930,8931..8948,8949..8955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8963..8979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8968..8990
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8987..9003
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8987..9003
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgaag tgattggcgg cagccctaac aacgaggccc
     1381 ccaactctgc ccggcaccac ctgagaaatg gcttccccgg caagaacgac gccctgcccc
     1441 acgaggaacc caacaacctg gaaggcaaga atgaggccag cgaccagtgc gacaccatca
     1501 acctgggcca agtgaccgag aaagagaaga aaaccatcga gcaggccagc gtgcaggccc
     1561 aggatgccac aaagcccgag gccaacaacg ccgagcagat ccaggccgag ctgcagaaag
     1621 tgaaaaccgc caaggacgag agcgccaccg ccgccaaaga tgccgagaca gccaagaaaa
     1681 acgccgtgga cgccggcaag ggactggatg ctgccaaggg cgccatcaag aaggccgaag
     1741 aagccgccgc tgaggccaag aagcaggccg gaattgccga aaaggccgag aaggatgccg
     1801 aggccgctgg caagaaggac aagctggagg acgtgaacag ccaggtgcag atcgccgtgg
     1861 aagccagcac caaggccaag gacaagaaaa ccgaggccga gattgctgtg gaaatcgtga
     1921 aggccgtggt ggccaaagag gaagcccaga aggcctctga cgaggcccag aaagcctgcg
     1981 agaaggctca gaaggcccac gccaaagctc agaaagctag cgacaccacc aagaccgtgg
     2041 aaaccttcaa gaccaacgcc gaagccgctg ccaaaaacgc caaagagaag gccggcaacg
     2101 ccaacaaggc cgccacagaa gccgaaagcg ccaacgagct gagcgtggcc aagcagaagg
     2161 ctaaggacgc cgaggaagct gctaaagagg ccaaaaaaga acaagtgaaa gccgagatcg
     2221 ccgccgaggt ggcaaaggcc aaagtggcta aagaagaggc cgacgccgcc cagaagaagg
     2281 ctgaggccgc caaaaagatc gtggacaaga tcgcccagga caccaaggtg cccgaggctc
     2341 agagagaagc caagctggcc acacagaccg ccagcaaagc caccgaagct gccacagagg
     2401 ctggcaaaaa agcccaggaa gccgaagagt ccagcaaaga agccgaggaa aaagccgaaa
     2461 ccagcgacgc cgtgaagggc aaagccgatg ccgccgaaaa agctgccggc gaggctaaaa
     2521 aggccagcat cgagacagag atcgccatcg aagtggccaa agccgaggtg ctgaatgccg
     2581 aagtgaagaa aaccgctcag gaagctgaga aggacgccac cgaggcaaaa gagcaggctg
     2641 agaaagccaa ggccgctgcc gaagaggcta agacccacgg cgagaaagca gagaaagtgg
     2701 gcgagagcac aaaggcccac tccgatgagg cccagcagga aaacaagaac gccaaggatg
     2761 ccagcgaaga ggcagagaac agagccgtgg atgccctgga agaggcctat gcagtggaag
     2821 cccacctggc ccggacaaag aatgccgcag agtccgccaa gtctgccacc gacatgagcg
     2881 agctggaaaa agcaaaagaa gaagccattg acgccgccaa tatcgcccac cagaagtggc
     2941 tgaaagccac ccaggccgct accattgcca aagaaaaaaa agaggccgct aaggtggccg
     3001 ctgaaaaggc ccagaccgcc gctaacgtcg tgaaggataa ggctgctaag gcagaggcaa
     3061 agaaggcaga aaccgaagcc gtgaaagcag ctgtggaagc tcgggccgct gctgaggaag
     3121 ctaaacagga agcagcaaaa gtgggagcct ccaaagagcc ccaggaaact aagaacaagg
     3181 ccaatgtgga agcagaggcc accggaaacg aagccaaaaa ggctgaagat gccgctgagg
     3241 aagccaaaga ggccgccaag aaggctaacg aggccaccga cgccaacgtg gccagaagcg
     3301 aagccgacaa ggccattgcc gctgctaaga aagcaaaaaa ggcacgggaa aaggctgcct
     3361 acggcctgct gaaaacaaag aaccagtacg tgctggaacc cctggacatc agccctgaga
     3421 gcgccgacaa cattgcctct aaagaggaac aagtgaaaga agagatggaa gatcagggcg
     3481 acgaggacag caatgaggct gaggtggaag aagccctgcc caacggcgct ggcgcacagg
     3541 aagaggatgt gaacctggaa atggacgacg aagaggaagt ggaagaggtg gaagaaaacg
     3601 tggccaccaa ccagcagacc ggcggcaagc gcgagaagcg gaacaccaac gacgctgtgg
     3661 acgacaccaa tgccgacaag cagttcggcg acgagttcga cacctacaac gacatcaaga
     3721 aagtgacaga ggccctcgtg aagtccatga ccagcctggt gtccgacgac cccagcgtgg
     3781 gagataccat caacgagttt ctgagcgaca tgaaccacct gtttctgtcc tggggcgcgc
     3841 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     3901 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     3961 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     4021 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     4081 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     4141 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     4201 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     4261 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     4321 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     4381 ccaaagggtt gaattccgga tcactgcatc atattctgga tgcacagaaa atggtgtgga
     4441 atcatcgtta agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     4501 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     4561 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     4621 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     4681 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     4741 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     4801 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     4861 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     4921 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     4981 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     5041 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     5101 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     5161 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     5221 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     5281 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     5341 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     5401 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     5461 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     5521 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     5581 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     5641 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     5701 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     5761 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     5821 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     5881 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     5941 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     6001 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     6061 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     6121 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     6181 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     6241 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     6301 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     6361 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     6421 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     6481 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6541 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6601 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     6661 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     6721 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     6781 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6841 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6961 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7021 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7081 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7141 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7201 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     7261 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7321 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7381 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7441 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7501 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     7561 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7621 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7681 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7741 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7801 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7861 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7921 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7981 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8041 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8101 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8161 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8221 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8281 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8341 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8401 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8461 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8521 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8581 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8641 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8701 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8761 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8821 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8881 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8941 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9001 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     9061 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
//
