LOCUS       sequence-120616-        7671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax MSP5 ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MSP5-bio
FEATURES             Location/Qualifiers
     source          1..7671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3051..3106
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3105..3124)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5342..5360)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5428..5532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5533..5601,5602..6393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5751..5770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6564..7152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7053..7072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7306..7323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7440..7461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7476..7481,7482..7499,7500..7506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7514..7530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7519..7541
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7538..7554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7538..7554
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccagac ctctggaagt gtctctgtgg ggccagggca
     1381 atgcccacct gggcacacag acaagccggc tgctgagaga gagcggcaga aacggccagg
     1441 ccaacagagt gaaccaggcc gatcaggctg accaggtggc cagccctcct atcagcggca
     1501 aagagcggag aagaggcatc ggcatgacca gcaacctgca gctgctgagc ggcgaggacg
     1561 agaaggacag cacaagcgag gaagccccca acctggaagg caaggacaac gccgatgccg
     1621 gcaaggacgg cgagaaagag cctagcgaga agcagagcgg cgacgtggac cctaccgtga
     1681 ccgatgccga gagagccaag gacgaaaacg ccgccgtgtc cgaagaggaa cagatgaaga
     1741 ccctggactc cggcgaggat cacaccgacg acggaaatgc cgatggcgga cagggcggag
     1801 gcgacggcaa tgacgagaat cagaagggcg acggaaaaga gaaagagggc ggcgaagaga
     1861 agaaagagga tggcaaagac gaccacgaga aaggcgagaa gggcagcgag ggcgagtccg
     1921 gcgaaaagga tgaagccgcc cctaaaggcg acgccgccga gaaggataag aagctggaaa
     1981 gcaagaccgc cgacgccaag gtgtccgagc acaaggccga cgatgccaac cctggcggca
     2041 acaaggatag ccctgagggc gagagcccca aagagggcaa ccctgacgac cccagccaga
     2101 agaatcctga ggccgctggc gacgacgaca gcagactgca cctggacaac ctggacgaca
     2161 aggtgcccca ctacagcgcc ctgcggaaca acagggtgga aaagggcgtg accgacacaa
     2221 tggtgctgaa cgacatcatc ggcgagaacg ccaagagctg cagcgtggac aatggcggct
     2281 gtgccgacga ccagatctgc atccggatcg acaacatcgg catcaagtgc atctgtaaag
     2341 agggccacct gttcggcgac aagtgtatcc tgaccaagag cagcggcgcg ccgtcgacct
     2401 ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca ctcaaccttg
     2461 gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct tcttcccagt
     2521 cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct actagcaacc
     2581 ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag gtctcccttc
     2641 agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg tatcaggaag
     2701 tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc tgtgaggtga
     2761 tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag gaggccaggg
     2821 tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg tggcaatgtc
     2881 tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta tccaaagggt
     2941 tgaattccgg atcactgcat catattctgg atgcacagaa aatggtgtgg aatcatcgtt
     3001 aagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     3061 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3121 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     3181 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     3241 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     3301 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     3361 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     3421 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     3481 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     3541 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     3601 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     3661 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     3721 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     3781 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     3841 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     3901 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     3961 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     4021 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     4081 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     4141 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     4201 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     4261 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     4321 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     4381 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     4441 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     4501 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     4561 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     4621 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     4681 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     4741 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4861 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4921 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     4981 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     5041 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5101 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5161 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     5221 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     5281 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     5341 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5401 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5521 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5581 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5641 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5701 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5761 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5821 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5881 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5941 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6001 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6061 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6121 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6181 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6241 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6301 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6361 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7141 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7201 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7261 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7321 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7381 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7441 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7501 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7561 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     7621 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg a
//
