LOCUS       sequence-120624-        7938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax MSP10 ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MSP10-bio
FEATURES             Location/Qualifiers
     source          1..7938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3318..3373
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3372..3391)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5609..5627)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5695..5799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5800..5868,5869..6660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6018..6037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6831..7419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7320..7339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7573..7590
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7707..7728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7743..7748,7749..7766,7767..7773)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7781..7797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7786..7808
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7805..7821
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7805..7821
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcagctg tgcatgtgtc tgccaacgaa ctgaacggcg
     1381 ccgatggcgg cgacgtgccc aaccagaagg acatcaccaa ggactacagc atcttcgagc
     1441 ggatcaccag cgagggccag tctgccagcg gcaaggacga tagccctatc agccagagca
     1501 acacccccca gggcgaagag gcctccgatg gcaagcaggg caataccccc ctggacgaga
     1561 agaactccgc caaggacgtg gaagcccact tcatccggga aggccacgac aaagtgaccc
     1621 acagcgacgt gggcaccgag gaaggcaaag ccacaggcca cgtgcagaag aacgccaacc
     1681 tgcggagcac cagctacttc agtacgcagg gcgccgtgtc ccaggcctac aacttcgtgc
     1741 aggaaaacca cccccagctg gacaacaacg gcgccaacgt ggaacaggtg gccagaggcc
     1801 aggatgatgt gggcagcacc gagaacggcg agggcaattc tggcggcgag ggaaatcctc
     1861 tgggcagcga taagcctggc ggcaagcctg aggacgccag caaggatacc cccggcaaca
     1921 accccgagga aagccccaac cggaaccagg aaaagggcga gaaagagaag aaagagcgcg
     1981 gcgacaccga caaccccaac agaggcaaga ccagcgagaa ggccgatcag cagggcggca
     2041 accaccctaa tggcctgagc cccgatgaga agaacaaccc taagacccac aacaaccacg
     2101 ccgagagcat caccaacccc ggggatgtgg gagccctgga cggcgaagaa aatggcgagg
     2161 gcgacgacca gaccggcatc agccctacag aggaacaccc tacaggcgac gcccctccta
     2221 gcgaccatgc cgagaagatc aagaacaccc tgctgaaaga gggcatcgac ctgaaagaaa
     2281 ccaccagcat gatcgacaac gccgtgtaca acatggaaca gttcatcctg aaaacaaagt
     2341 tctacaccac cgccatccgg aatttcgtgc acttcaaagt gaatcacatc tgcgagtaca
     2401 gcaagtgcgg cgccaatgcc cggtgctaca tcgtggaaaa ggacaaagag gaatgccgct
     2461 gccgggccaa ctacatgccc gacgatagcg tggactactt caagtgcatc cccatggtgg
     2521 aaaaagactg cagcaaagag aacggcaact gcgacgtgaa cgccgagtgc agcatcgaca
     2581 agaacaagga tatcaagtgc cagtgcaagt tcaactatat cggcgacggc atcttctgcg
     2641 tgatgggctc tggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     2701 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     2761 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     2821 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     2881 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     2941 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     3001 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     3061 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     3121 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     3181 ccaagatcca ggttttatcc aaagggttga attccggatc actgcatcat attctggatg
     3241 cacagaaaat ggtgtggaat catcgttaag tctagacacg tgattaatta aggatccccc
     3301 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3361 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     3421 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     3481 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     3541 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3601 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3661 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     3721 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3781 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3841 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3901 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3961 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     4021 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     4081 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     4141 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     4201 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     4261 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     4321 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     4381 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     4441 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     4501 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     4561 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4621 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4681 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     4741 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4801 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4861 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4921 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4981 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     5041 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5101 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     5161 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     5221 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     5281 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     5341 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     5401 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     5461 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5521 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     5581 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5641 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5701 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5761 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5821 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5881 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5941 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6001 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     6061 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6121 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6181 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6241 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6301 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     6361 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6421 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6481 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6541 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6601 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6661 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6721 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6781 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6841 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6901 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6961 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7021 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7081 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7141 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7201 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7261 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7321 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7381 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7441 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7501 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7561 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7621 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7681 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7741 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7801 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     7861 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7921 agaactggta ggtatgga
//
