LOCUS       sequence-120626-        7581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax P12 ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: P12-bio
FEATURES             Location/Qualifiers
     source          1..7581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2961..3016
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3015..3034)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5252..5270)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5338..5442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5443..5511,5512..6303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5661..5680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6474..7062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6963..6982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7216..7233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7350..7371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7386..7391,7392..7409,7410..7416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7424..7440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7429..7451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7448..7464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7448..7464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcattca cccacacctg tgacttcaac gacgagctga
     1381 gcctggaatt cgacgagcgg cagatgacca acggcgagca gatctgtacc ctgaagcccg
     1441 acgtgctgga caaggtcgtg atcaagtgtg gcgccgagcg gaagaactac gagctgctgc
     1501 ccaacaactg cttcgaacag gtgttcacca gcaagagcat cgaggacgcc aagaacgccc
     1561 acaacgccca taacgtgcac aatgtgcaga atgcccagca gctggtggaa tacctgcatg
     1621 gcgccgctgc catcgtgaag cggaagcaga atgaggccaa cgccgacgag aagaaaagct
     1681 acgacgacgt gtccttccgg gtgcccccca acctggaaaa agagcagaaa gccctgtact
     1741 gcgtgtgcca gaacaaggcc aagatcagag tgcggaagcg gagcggcgag gaatacgaca
     1801 aagagatctt caacctgggc atcatcgagg tgctgatccc cagcctgccc aagaagatcg
     1861 acggctgcga cttcaccaag aacaccgccc ctctgttcac caagggctac gacgccaact
     1921 tctacaagac catcgagaac aaggacgacg tgatctgcaa agtgaaggcc accgagggca
     1981 agctgatcgg cttcaagtgc cctgccgact acgccatcga gcccgaagag tgctttctgc
     2041 aaggcttcaa tctggccggc aagaaagagc agctgcggac caagatcgag ctgaccgacc
     2101 tcgtgatgga ccactacaac aagatcttct acgccagagt gccccagcgg atctaccaga
     2161 atatgcactt cttctgcgcc tgcgtgctgg aagagaagcg gctggtggcc cacttcgagt
     2221 tcgccaccac ccccgacgac cagaactccg tgtctgaacc cagagccgcc aacgtgctgc
     2281 agggaagatc tgcaggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     2341 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     2401 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     2461 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     2521 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     2581 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     2641 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     2701 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     2761 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     2821 actccaagat ccaggtttta tccaaagggt tgaattccgg atcactgcat catattctgg
     2881 atgcacagaa aatggtgtgg aatcatcgtt aagtctagac acgtgattaa ttaaggatcc
     2941 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3001 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     3061 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     3121 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     3181 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     3241 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     3301 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     3361 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     3421 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     3481 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     3541 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     3601 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     3661 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     3721 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     3781 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     3841 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     3901 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     3961 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     4021 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     4081 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     4141 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     4201 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     4261 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     4321 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     4381 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     4441 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     4501 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     4561 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     4621 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     4801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     4861 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     4921 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     4981 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     5041 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     5101 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5161 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     5221 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5281 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5341 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5401 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5461 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5521 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5581 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5641 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     5701 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5761 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5821 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5881 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5941 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     6001 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6061 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6121 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6181 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6241 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6301 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6361 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6421 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6481 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6541 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6601 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6661 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6721 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6781 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6841 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6901 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6961 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7021 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7081 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7141 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7201 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7261 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7321 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7381 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7441 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     7501 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     7561 cgcagaactg gtaggtatgg a
//
