LOCUS       sequence-120642-        7428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax ARP ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: ARP-bio
FEATURES             Location/Qualifiers
     source          1..7428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2808..2863
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2862..2881)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5099..5117)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5185..5289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5290..5358,5359..6150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5508..5527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6321..6909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6810..6829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7063..7080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7197..7218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7233..7238,7239..7256,7257..7263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7271..7287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7276..7298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7295..7311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7295..7311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaaga gcatcttcag aaagcggaag atcatctacg
     1381 gcagccccca tctgctgaac gcccccgatg atgaggccat caagttcccc cccctggaca
     1441 acctgaaggc caaagagagc cccagcagca acgacgacgg cgtgtacgcc aagggcagcc
     1501 actttgtggg caagggctcc accagcaacc tgggcgacgt gaagaacggc gccttcatcc
     1561 tgctgagcga gaaagtgaag gacatcagct ccgacgagga agaggaagaa gaggacggcc
     1621 acgacaacgc cgaccacgtg aacaacaacg tgaacagcca caaccacgcc gacgacacca
     1681 gcgagagcga gtccgaggac gagcacaaca acaatgagaa ggatcacgcc gagggcgacc
     1741 acgagcatct gcacaatggc tacagccccg acgagaacgg caactacgac gtgaacgggg
     1801 atgtgaatgg ggacgtgaac ggcaatgtga acggcgccat gaacggcaac atgaatggaa
     1861 atatgaatgg gaacatgaac gggaatatga acggaaacat gaacgggaac atgaatggca
     1921 acatgaacgg caacatgaat gggaacaaca acaacatgaa caacaatatg aacaataaca
     1981 acaacaacaa tatgaatggc aacggcgcct tcaccgccct gcctccacct ccaccacctg
     2041 tggtgcatca tccaccccct gcccctatga ccccttctgg cattgtgggc cacgtggtgt
     2101 ccaacgtgtt ctctgccggc ctgaagctgt tcggagtgcc tggcgcgccg tcgacctcca
     2161 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     2221 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     2281 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     2341 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     2401 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     2461 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     2521 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     2581 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     2641 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     2701 attccggatc actgcatcat attctggatg cacagaaaat ggtgtggaat catcgttaag
     2761 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     2821 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2881 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     2941 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     3001 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     3061 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     3121 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     3181 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     3241 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     3301 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     3361 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     3421 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     3481 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     3541 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     3601 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     3661 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     3721 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     3781 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     3841 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     3901 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     3961 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     4021 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     4081 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     4141 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     4201 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     4261 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     4321 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     4381 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     4441 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     4501 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4561 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4621 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4681 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     4741 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     4801 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4861 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4921 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     4981 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     5041 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     5101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5281 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5341 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5401 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5461 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5521 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5581 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5641 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5701 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5761 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5821 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5881 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5941 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6001 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6061 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6121 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6421 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6961 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7021 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7081 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7141 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7201 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7261 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7321 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     7381 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatgga
//
