LOCUS       sequence-120646-        9795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax RIPR ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: RIPR-bio
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5175..5230
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5229..5248)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7466..7484)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7552..7656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7657..7725,7726..8517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7875..7894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8688..9276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9177..9196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9430..9447
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9564..9585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9600..9605,9606..9623,9624..9630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9638..9654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9643..9665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9662..9678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9662..9678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaatg tggtggaaga tgacatcttt taccagaaag
     1381 gcgaggtgga aaagctgacc ttcagcctgg accacagagt gcgggacaag agcaagaaag
     1441 aacgggtgga ctacaccaac ggcgagaacg cctacgccta cgtggacaag gccattgcca
     1501 aggtgttcac cctgaccagc gaggccgaga tcaagaagtt ctttagcctg aacaaggacg
     1561 tgaaaacctg cgactacctg atcgcccagg gctccctgaa gaaggacctg cagaaaaaca
     1621 agtgctaccg caagaccttc tgcggcgtga tcccccacag agagtacatc cctggcggcg
     1681 gaagcagcaa caagagagag agcgacctga tccactgcgc ctacatgaac gacgcccaca
     1741 tcgtgatcta ccacgtgggc agaccccatc tgctgaagcc caacgtggtg tacgaggaac
     1801 tgttttttca agagtccgag aagggcgtga tcagctgcca cgccatgaac atcaacctga
     1861 gatacgtggg catccacacc agcggcaaca acgcctgcat ccaggactac ctgcaggaac
     1921 acctgaaaga gctgtgcaac gagaagaaag cctgcgagat cgacttcaag aacgtgaaga
     1981 agggcaacag ctgcagcctg gaaaacaact ttctgatcca cgtgaactac gagtgcgtgg
     2041 acagctgcaa ccagaaagag aaccaggcct gcgacatcta caatggcgag ggccggatga
     2101 ccacctgtag atacggctac aacatgctgc cccggatcaa cgacttctgc gagaagaact
     2161 acgcctgcaa cagcagagtg tgcagcgtga accagttctg cgacgaggcc accgagagct
     2221 gcgtgtgcaa gacaagcctg ctgcccgtgg aaaagaccca ctgcctgtac acccacgtgt
     2281 gcgacgccat caagtgcccc gaggatgcca catgcgtggt ggaacggaac agcaaaaagg
     2341 ccgagtgcag atgtgacgag ggcaagtacc tgcacaagaa tgagtgctac aatatggaag
     2401 aactggaacg gctgatcaag gccgagacaa ccccccacga gaagcagtac aagaaggatc
     2461 tgttcatccg cagcgccctg aagcccgagc acatctacat gcactgcgag gaaggctaca
     2521 gcatccacgt cgtgaacgcc gccctgagct gctaccacat cagcttcaaa gagaacaaga
     2581 tcaagtacat caccgaccgg ctgagagagg cctgtaacgg cagaggcaga tgcgccttcg
     2641 gcaacagcgt ggaccaggtg gtgcctcctc tggacagcgc caatatgtgc ggcaccaagg
     2701 gcaccatcta ccagtacgag tacgtgtgcg tgcggggagg cccttctcct cctgtgcctg
     2761 tgatggctca gctgggcaga gatccacccc ccaagagcct gaccaaagag aagggggcca
     2821 tcttcagaag ccggttccac agccagatcg agtgcccagg cggcaccatt accgtgaaca
     2881 aggccttcct gaaggccggc gacggctgcg aggatctgga cctgaccaac tccgtgaaag
     2941 agtactgcga ccagctgtcc ttctgtgacg tgggcctgac ccaccacttc gacacctact
     3001 gcaagaacga ccagtacctg ttcgtgcact acacatgcga ggacctgtgc aaaacctgtg
     3061 gccccaacag cgcctgctac ggcaacaagt acaagcacaa gtgcctgtgc aacagcccct
     3121 tcgagtccaa gaagaatcac gccatctgcg aggccagggg cagctgtgat gcacaagtgt
     3181 gcggcaagaa ccagatctgc aagatggtgg acgccaaggc cacctgtacc tgcgccgaca
     3241 agtaccagaa tgtgaacggc gtgtgcctgc ccgaggacaa gtgcgatctg ctgtgcccct
     3301 ccaacaaagc ctgcctgctg gaaaacggca agaaaatctg caaatgcatc aacgggctga
     3361 ccctgcagaa cggcgagtgt gtgtgtagcg acagctccca gatcgaagag ggacacctgt
     3421 gcgtgccaaa gaacaagtgc aagcggaaag aataccagca gctgtgcacc aacgagaaag
     3481 aacactgcgt gtacgacgag cagaccgaca tcgtcagatg cgactgtgtg gaccacttca
     3541 agcggaacga gcggggcatc tgcatccccg tggactattg caagaatgtg gcctgcaaag
     3601 agaatgagat ctgtaaagtc gtgaacaacg cccctacctg cgagtgcaaa gaaaacctga
     3661 agcggaactc caacaatgag tgcgtgttca acaacatgtg cctcgtgaac aaaggcaact
     3721 gccccatcga cagcgagtgc atctatcacg agaaaaagcg gcaccagtgt ctgtgccaca
     3781 agaaaggcct ggtggccatc aacggcaaat gcgtgatgca ggacatgtgc cgcagcgacc
     3841 agaacaaatg cagcgagaac agcatctgtg tgaaccaagt gaacaaagaa cccctgtgca
     3901 tctgtctgtt caactacgtg aagtccagaa gcggcgacag ccctgagggc ggccagactt
     3961 gtgtggtgga caatccttgc ctggcccaca acggcggctg ctccccaaat gaagtgtgca
     4021 cctttaagaa cggcaaggtg tcctgcgcct gcggcgagaa ttaccggcct agaggcaagg
     4081 acagccctac cggacaggcc gtgaaaagag gcgaggccac aaagagaggc gacgccggac
     4141 agccaggaca ggcccatagc gccaatgaga atgcctgtct gcccaagacc tccgaggccg
     4201 accagacctt cacattccag tacaacgacg acgccgccat catcctgggc agctgcggca
     4261 tcatccagtt cgtgcagaaa agcgaccaag tgatctggaa gatcaacagc aacaaccact
     4321 tctacatctt caattacgac taccccagcg agggccagct gtctgctcag gtcgtgaaca
     4381 agcaggaaag cagcatcctg tatctgaaga aaacccacgc cgggaaggtg ttctacgccg
     4441 actttgagct gggacaccag ggctgctcct acggcaatat gttcctgtac gcccaccggg
     4501 aagaagccgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     4561 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     4621 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     4681 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     4741 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     4801 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     4861 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     4921 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     4981 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     5041 agatccaggt tttatccaaa gggttgaatt ccggatcact gcatcatatt ctggatgcac
     5101 agaaaatggt gtggaatcat cgttaagtct agacacgtga ttaattaagg atcccccgac
     5161 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     5221 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     5281 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     5341 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     5401 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     5461 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     5521 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     5581 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     5641 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     5701 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     5761 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     5821 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     5881 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     5941 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     6001 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     6061 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     6121 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     6181 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     6241 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     6301 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     6361 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     6421 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     6481 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     6541 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     6601 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     6661 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     6721 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     6781 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     6841 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     6901 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6961 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     7021 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     7081 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     7141 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     7201 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     7261 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     7321 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     7381 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     7441 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     7501 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7561 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7621 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7681 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7741 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7801 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7861 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     7921 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7981 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8041 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8101 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8161 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     8221 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8281 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8341 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8401 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8461 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8521 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8581 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8641 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8701 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8761 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8821 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8881 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8941 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9001 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9061 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9121 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9181 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9241 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9301 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9361 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9421 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9481 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     9541 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9601 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9661 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     9721 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     9781 actggtaggt atgga
//
