LOCUS       sequence-120648-        9231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax RhopH3 ectodomain in mammalian cells.
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: RhopH3-bio
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4611..4666
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4665..4684)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6902..6920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6988..7092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7093..7161,7162..7953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7311..7330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8124..8712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8613..8632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8866..8883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9000..9021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9036..9041,9042..9059,9060..9066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9074..9090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9079..9101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9098..9114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9098..9114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccagag agtacttcag cggcttcgtg aacaagaagc
     1381 tgaagaacct gctgcagtgc aacctggccg cctactacaa cctgagagac aacggccccg
     1441 accccaacgc cttcctggat tttgtgggcg agcccgagca gttctactgg ttcatcgagc
     1501 actacctgag cgtgcccttc agcatccccc ggaacctgaa gaacaacaag gcccacagct
     1561 actggtcctg catcaaccgg gcctgggtgt ccgagttcct gcggaagtac gaggaacccg
     1621 acatcaacga gctgatgaag ttcctggaca aagaacagcg ggtgtacttc tcctacacct
     1681 tccagaacat cgagcccgtg gccaagtaca ccagcttccc catcaaagag ttccacaagt
     1741 actgcgtgct gccccccctg atcgagacaa acatcaagca gaaggacaag ggcggcaatc
     1801 tggccttcca gctgaaccag gccgagtaca agatctacct gtccagcgtg ggcaccccca
     1861 ccagcgccct gaagaatctg tacctgaaca tggaagatgg cgagcggaag aacatcgtga
     1921 aaaccatcgt ggaaaacgag ggcagcaaca acgtgttcgt gaactgcccc gtgtacgacc
     1981 tgaagctgca ctacaacaaa gagtgcggca gccagcccaa cgtgctgaag tgcctggatg
     2041 actatatcaa gcacctgtgc gagcggcgga tcgcccacag agagaagggc accttctgcg
     2101 acgacctgct gttcctgttc gacgccctga aagaacccta cgtggaaaat ttcaagaagt
     2161 ttctgagccg ggacgacgtg cacctcgtga agcctcagtc tgtgtggggc atccccctgt
     2221 tcaccaccta caagcccaga gatctgaaaa accccaagaa caacgtgccc ctgaacgtgt
     2281 tcaaggtgct gaacagcaag aacaaactgt tcctgagctt cttcgacgag atccccaaga
     2341 gcccctacta tatggaagag tcccagcccc tcgtgaaact gagcgacttc gccagcagca
     2401 tcttcgacaa gctgcaccgg ttcttctatg tgttcaagaa gaagggcaac cagatcagcc
     2461 ccgtgtccgt gaaagagctg agccacaata tcgccgattt cagcttcaag cacgacacct
     2521 ccaacatcga gtgcaagaaa gtgaagaagt ccctgaacct ggaactggaa gtggaagtgg
     2581 ccaagggcgt ggccgccgag aagatctgca atctgatcga gggcttcgtg ctgaccaagg
     2641 gcaagaagga taagaccatc cggggcgagg tggacgatgt gcacaagggc ttccggatcc
     2701 agtgcatcct gatcgccacc cacgtggaag ccctgaatat cgtgcgccag ctgctgaata
     2761 tggaatccat gctgagcctg accagataca ccagcctgta cctgcacaag ttcttcaaga
     2821 gcgtgacagc cctgaagggg aacttcctgt acgagaaccc cagcgccatc cggcacgcca
     2881 gagcttgtgg cagagctgtg ctgcatgtgc ccgccgtgct gtaccggcgg aatatctacc
     2941 tggccgagac attcctgagc ctgtatctgg gcctgagcaa cctggtgtcc agcaacccca
     3001 gcagcccatt cttcgagtac gccatcatcg agtttctggt gtcctacttc aacaagggca
     3061 gcgagaaatt cctgctgtat ctgttctcca tcgtgtctgt gctgtacatc aacatctact
     3121 actacgagca gctgtactgc caccaccgcg agcagtttga gctgctgcgg agcaagatga
     3181 tccaccccaa cattgccgac cggatcctgg aaaacattaa gaccctgatc aagaaccccc
     3241 ggtacatgaa gatgcggacc ttctacatga agttcgacaa cgaggacatg ttcgataagc
     3301 ggaaggtgtt cgaagtgctg tactccttcg atgagttcct gtcctccagc gacgctcagc
     3361 agaaagccaa gatgcaggat atcagcgacg agagcgtgga cctggatacc accaacgacg
     3421 gcatcggcct gcggaaagag gacgccctgt ttgagtctga gcagcagggc agcatggaat
     3481 ctatcgagga catcgacgag ggcctggaag gcgtggaccc caaccagcag aagaaaaccg
     3541 ccgagtatct gaagatggtg cccgacgagg acaaggccaa cctggctgac cggaacaaag
     3601 agctggaact ggacctgtac aagtacatcg gcaccctgaa cgaggcctct gccggcgtgg
     3661 gaaccgtgtc tacacacagc cctgctaccg ccgccagaat gggactgaat gctggcggag
     3721 gcagagccgt gcggcctgct gctaaaggac tgcaccagcg gatgggccag atgggcaagg
     3781 acctgaagag gatgaagggc aaggatacca gcaagctgcg gaagggggtg gacttctacg
     3841 agagcagcac ctccctgaat cagatctccc ctggcgagag cgccacagag ggcaagggct
     3901 ctagcaccaa tgtggccacc gagggcacca gcccatctga aacaggcgcg ccgtcgacct
     3961 ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca ctcaaccttg
     4021 gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct tcttcccagt
     4081 cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct actagcaacc
     4141 ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag gtctcccttc
     4201 agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg tatcaggaag
     4261 tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc tgtgaggtga
     4321 tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag gaggccaggg
     4381 tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg tggcaatgtc
     4441 tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta tccaaagggt
     4501 tgaattccgg atcactgcat catattctgg atgcacagaa aatggtgtgg aatcatcgtt
     4561 aagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     4621 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     4681 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     4741 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     4801 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     4861 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     4921 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     4981 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     5041 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     5101 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     5161 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     5221 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     5281 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     5341 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     5401 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     5461 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     5521 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     5581 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     5641 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     5701 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     5761 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     5821 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     5881 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     5941 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     6001 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     6061 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     6121 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     6181 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     6241 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     6301 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     6361 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     6421 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     6481 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     6541 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     6601 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     6661 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     6721 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     6781 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     6841 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     6901 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6961 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7081 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7201 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7321 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7621 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7921 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8821 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8881 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8941 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9001 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9061 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9121 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     9181 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg a
//
