LOCUS       sequence-120652-       10047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax CLAG (PVX_121885) ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: CLAG-bio
FEATURES             Location/Qualifiers
     source          1..10047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5427..5482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5481..5500)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7718..7736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7804..7908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7909..7977,7978..8769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8127..8146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8940..9528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9429..9448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9682..9699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9816..9837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9852..9857,9858..9875,9876..9882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9890..9906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9895..9917
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9914..9930
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9914..9930
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcctacg tgaacgagaa agagaacctg gaagaactga
     1381 aatacgtgat cgagggcaaa gagatgttcg acaacctggc cggcctggaa aagctgatca
     1441 tcgagacact gaagttcgac aaagtgcggc tgcccgtgct gaaccccagc ctgaaagaat
     1501 acctgaacat ggccaacttc gccatcgtga agcacaacat caacagcgag aacgtgatca
     1561 acctggtggt gcccaccccc gacgccgaca tccacgacct gattaagtac gagcacgcca
     1621 ccaagcagca gatcatccag aagtacgact ccgagaacag cgactctatc aagcggaagt
     1681 tcctgctgct gaaggccctg aaaaccacca agctgatgct gatccctatg tacgcctacg
     1741 aaaagaccaa ggacctgaag aaggctctgg aagaactgaa cgccgtgttc ttcccaaaga
     1801 acgaggaact ggacaaagag aacagctacc agtacagcaa gagctacttc gagaagatca
     1861 tcaactacat caacggcgtg aagcggagag agagcaagga caacgcctac agcaccatca
     1921 tcatcggcga ggacctgagc aagatccagg aaagcagcga cctgttcttc accaccaacg
     1981 acaacatcga gttcatgagc aagctggaca agatcgccag ccacttcgga atcgccatgt
     2041 acaacatcgt gggcagcaat ctggtggccc tgggacactt cgtggtgctg cagctggccc
     2101 tgaagaaata cgaccagttc tttaagttcg gcaagatgcg gttcttcaac tggcagaaga
     2161 tcctgagctt cagcgccacc gaccggttca gagtgctgga cgccatgtgc aacgtgcagg
     2221 gcgtgcagga tgtgcagacc aagcggcgga tcgtgtacct gaagtccgac cggaccgcca
     2281 gcttcgacga gtgcaatatc ctggaattcc tggtgcatca cttcaacaag taccagaaca
     2341 gcctggtgtc cggcctgtac caggaagatt tcaagaccca ctacctgcag gaacacagcg
     2401 acatccggaa cgagttcttc aagtttatgt gcaatgacat caaccactgc aacatctaca
     2461 gcagcaagct gttcctgagc gagagcgaca ccatgcggcc cctgaacagc gataccccct
     2521 actacaaggc catcagcgcc tacaacctgt acaccaacta cctgtacttt acccggcact
     2581 acaacgagtt cacccccaac catatcctgt acgtgcactt cctgaatctg gccggcatcc
     2641 tgaacaacga gaacaaggcc ttcgtgacca gcctgtacct gcctggccac tataacgcca
     2701 tcgagctggc cttcgaccag gaatccctgc tgaccgacct gatcgagaac ctgctgcagt
     2761 gcgtggaaaa gtgcaacagc atcgacgagc cccagaagaa gtccgagaac gacgacagct
     2821 ccaactccga ctacggcgag agcaagagca ccaagtgcaa cctgtgcaag ggggtgttca
     2881 cctacatcaa tagcaagcac gaagaggccc cctccatgct gcagaaattc ttcacctttg
     2941 tgaccaagat cattaacatc aactccgtgt ccaccctcgt gcggaacctg ggcgtgtacg
     3001 aggaatacga caacttcctg accaacgata tcgactggta caaatttctt ctgctgctgc
     3061 ggctgaccag ctacaagaag atcgccgaga aggacgtggg cgaggccatg tacctggatc
     3121 tgaagaaaga ggacaagttc aacaaagcta tcgtgaccaa ctactggtat cccagctacc
     3181 tgaaaaaggc ctacacactg tatgtgcgga acaagctggc cattaacctg gtggaaaagc
     3241 tggaaaacct gctgagcaag ggggccattg agaagatgaa gaaaagcgtg cggtttctgc
     3301 tgcacgtgaa cagctttctg cagctggact tcttccacaa cctgaacgag cccggacccc
     3361 gcgaggacag aatgcaccct ctgagcatgg tgctggaaaa tcagttcgcc cagtggtcca
     3421 gcaacagcgc cctgggctac ttcttcctga actacgacga ccccaccacc cggaagcaga
     3481 tgcacgagaa gctgcggagc gaccggctcg tgatccccaa gttccacaag gtgtccgtga
     3541 tgctgaagaa tcacatcagc aaggcctacg agtcctactt caaccagcgg cacgtgaaga
     3601 atctgtacaa gaattacgac agcttcaata tcaacaacaa gatcatcctg atgaaggaca
     3661 gctacgagag ctatctggaa aactaccagg atatcatctt tctggccgac atcttcaaca
     3721 ttcggaagta cctgaccgcc acccccaagg ccaagcgggt gtccgacaga ctgtactact
     3781 acatccacaa catcctgggg aacagcgtga acttctataa gtacggcatg atctacggct
     3841 ttgtgattaa caaagaatac ttcaaagagg tggccaacga gctgttcagc atcttcaagc
     3901 tgaaccagca catctttacc gatatcagct tcctggaaac agtgtacctg ctgtcccgga
     3961 agatcgagag cagcttcaac gcccagcggc ggaacgacaa gatctctatc agcaacctgt
     4021 ttttcctgaa cgtggcctcc aactacgcca agatgagcaa ggccgagcgg ctggaagatc
     4081 tgaacaacgc catggccagc cggttcttta gcaagacctt ttttagcacc ttccagacca
     4141 tgttcgtgtc catgatcagc cggaaggtgg acaaactgga taagaagtat ggcaccgcca
     4201 ccatgctggg cgtgaccctg tctgagaagg cctttatgaa ctacgccttc atctactacg
     4261 gcagcatcat ggataatatc gccaactccc tggtgcccat ctatgccaag aagcccatcg
     4321 agcagctgaa atatggcaag atcttcatcc tgagcaacta cttcatgctg tccagccaga
     4381 tctttagcct gctgaacctg aacaatctgg cccagctgtg cgagtaccag gccatctcca
     4441 gctccgccta ctacagcggc aagaagctgg gccggttcat cgacaagaaa atgctgccca
     4501 tcgtggtgta ctacctgaag atgcgggtgg aaggcgtgaa aaccgacccc agcgagttta
     4561 ccaacgcctg gaccgtgatc aagcggcccg atttccaccc cgccatctgc atcaccgtgc
     4621 acatgatcac caatctgtac ttcgataccc ctatcgcctt cccattcagc atgggcgcca
     4681 agctgggcga gcagaccgaa catatgcccg ccctgaagcc cagctccaag cccttcgtgt
     4741 tcggcttcac caagatgctg ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     4801 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     4861 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     4921 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     4981 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     5041 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     5101 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     5161 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     5221 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     5281 agatggactc caagatccag gttttatcca aagggttgaa ttccggatca ctgcatcata
     5341 ttctggatgc acagaaaatg gtgtggaatc atcgttaagt ctagacacgt gattaattaa
     5401 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     5461 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     5521 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     5581 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     5641 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     5701 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     5761 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     5821 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     5881 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     5941 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     6001 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     6061 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     6121 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     6181 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     6241 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     6301 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     6361 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     6421 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     6481 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     6541 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     6601 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     6661 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     6721 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     6781 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     6841 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     6901 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     6961 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     7021 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     7081 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     7141 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     7201 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     7261 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     7321 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     7381 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     7441 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     7501 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     7561 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     7621 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     7681 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     7741 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7801 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7861 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7921 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7981 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8041 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8101 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     8161 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8221 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8281 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8341 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8401 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     8461 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8521 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8581 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8641 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8701 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8761 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8821 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8881 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9781 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     9841 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     9901 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     9961 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
    10021 tgcaggcgca gaactggtag gtatgga
//
