LOCUS       sequence-120654-        8052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax PVX_081550 ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: PVX_081550-bio
FEATURES             Location/Qualifiers
     source          1..8052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3432..3487
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3486..3505)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5723..5741)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5809..5913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5914..5982,5983..6774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6132..6151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6945..7533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7434..7453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7687..7704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7821..7842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7857..7862,7863..7880,7881..7887)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7895..7911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7900..7922
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7919..7935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7919..7935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccagag atgggagaag aaagtggatg ggcctgctga
     1381 gcagatggga ggccacacct tacggcggct ctgatgtggg atccgatgtg ggcctgggac
     1441 agacaggcgg aggaaaggcc gcccacatct tcgccctgaa gtccgccctg cagttcgcca
     1501 acggctacga ggaagtgcgg cagaagggca gatctggcag aagcggaaga agcgagcggc
     1561 gcgagcggag agaacgcgtg aaccctgtgt gcatcctgcc cagcgacatg ccccggttca
     1621 gaaccctgca cgagaaggtg gaagtggaag aggaactgct gctgcaggaa aagaccgccg
     1681 acgacatgaa cgtggccggc gacgccgaca acaccaacaa caaggacgag tacggccagc
     1741 ccctgatcaa gggccagaca gccacagaag gcgtgctgcc tcagcagacc gtggccacaa
     1801 aagtgtcccc cctgatcgag aagtccacca tcgaccacta cctggacgtg tgcacccaga
     1861 tccggaagaa aaccttcagc ttcaagaact gcgagatctt ctacgagagc cccgaagtga
     1921 ccctgtacaa gaacatcctg gaagataaga gcgagacacg ctacgacctg atcggctacg
     1981 gcaccctgaa cgacgtgtcc ctgtatggcg ccagccaggc cctgaacaac ctggatgtga
     2041 tcaaagagtg gaacaagaat atctacaaga tcaactacct gaaactgaac aaggccagca
     2101 tcctggagaa gtacgagaac gacgagaaga tcgacgccac caagtatatc ctggaaaaag
     2161 aacacctgag agagaaccgg cggtacatct acctgatcaa cggactgccc tggcccttcc
     2221 ggtcccacga taccgtgtac gagttctacc agaagtacat cgagaaccag aacatgctgc
     2281 tggtggccaa caaggccgtg aacgaggtgt tcagcgacaa cagctactac acccggatca
     2341 gagactacga gaacttcttc tgcatctacc ccaagagcaa gaacagctac gagaagggcc
     2401 tggactacgt gatcagcgtg tactacgacg tgaacatccc caagttcatc cggaacaaca
     2461 tcctgagcca gatcttcccc agcctgatct tcaacctgca cgaagtgtcc aagaccatca
     2521 ccgagaaagg actggccatg agcagcgacg acatcaagaa gaacgagctg cccttccagc
     2581 tgaaggacaa cttcgcccct ggcgaaggcg gagccggcca ggatggaaaa gaaggccagg
     2641 acggcaagag ccccttcttc ggcgctaccg tgctgagagt gatcttcgtg gaccctttcc
     2701 acttcatctg gactaccaac gtgaacttct ttaagaaaat cttcgtgatc gtgaccagca
     2761 tctttggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     2821 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     2881 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     2941 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     3001 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     3061 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     3121 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     3181 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     3241 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     3301 tccaggtttt atccaaaggg ttgaattccg gatcactgca tcatattctg gatgcacaga
     3361 aaatggtgtg gaatcatcgt taagtctaga cacgtgatta attaaggatc ccccgacctc
     3421 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     3481 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     3541 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     3601 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     3661 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     3721 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     3781 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     3841 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     3901 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     3961 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     4021 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     4081 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     4141 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     4201 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     4261 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     4321 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     4381 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     4441 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     4501 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     4561 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     4621 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     4681 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     4741 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     4801 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     4861 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     4921 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     4981 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     5041 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     5101 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     5161 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     5221 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     5281 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     5341 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     5401 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     5461 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     5521 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     5581 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     5641 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     5701 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5761 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5821 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5881 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5941 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6001 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6061 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6121 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     6181 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6241 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6301 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6361 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6421 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     6481 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6541 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6601 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6661 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6721 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6781 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6841 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7621 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7681 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7741 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7801 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7861 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7921 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
     7981 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     8041 ggtaggtatg ga
//
