LOCUS       sequence-120658-        9309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax PVX_084970 ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: PVX_084970-bio
FEATURES             Location/Qualifiers
     source          1..9309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4689..4744
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4743..4762)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6980..6998)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7066..7170
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7171..7239,7240..8031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7389..7408)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8202..8790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8691..8710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8944..8961
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9078..9099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9114..9119,9120..9137,9138..9144)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9152..9168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9157..9179
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9176..9192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9176..9192
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgata aggctgtgaa tctgggcaat gccctggctg
     1381 gcggcatcag cggaggaatc tccggcggaa tttctggcgg cctgtctagc ggactggcca
     1441 gcggaattag cggcggactg agcaatagcg ccgctgcctc tacagccctg ctgcatgccg
     1501 ctgtgggcaa gggccccaac atgcacactt gtcagtctgc cggctgcgcc agctacaaga
     1561 gcatcacccc atccgacgcc ggcgactgcc tgaacggctt catctgcaaa gagtgcaagc
     1621 ggacccacgc caagaacccc aacatctgct tctacagcag cctgcagggc ttcgagagcc
     1681 tgtacgaagc ccacctggaa gatttcaccc agcccacccc ctacgaccgg ttcaatgtgc
     1741 ccctcgtgaa gtccagcaag ggcgagaaca acagaggcga cgccagcagc gacagcggca
     1801 gagaagtgtc ccccaacgac gagagcggcg accacagaag aggcagcctg tctcagggcg
     1861 gcgacgacga tggcgagaaa ggcgacctgc agagaagcgg cagggatggc aaagccggcg
     1921 gaagcagatt ccccagagcc ctggaagaag aggaagagga agaagaagag gaagaggacg
     1981 acgacgacga cagcgccaaa gagaagagag gcggccacaa gggcggcaac tctccacagg
     2041 gcggaaacaa cggcgggaac aacttcgatg ccggctacga gacagagagc ttcctgcaga
     2101 aaagccccga ccacgtgcac cggaagggcg atctgcacaa gctggccaaa gagggcggaa
     2161 gagagcagca caccaacgcc cacatgcaca tccacaccca tatgcacaca catacccaca
     2221 acgagctgat gagcggcaaa gagggactgc tgagcagcgt ggaaacccac gtgcggctgg
     2281 gaatcagcga gggcggctat aatagaggcg ccagcgagag cccaggcaga cacagcggag
     2341 tgtcatctgg cgctagcgtg tccatgggaa cagccgccca tggcggaaca gctgccgagt
     2401 ccggctacag ctttgccgag agcgagcggg gcaaagaaaa gatcgtgtac aagcggctga
     2461 agatcagcct gaacaaccac gaggaatact tcaagagcaa gatgaacaag tgccacgtgg
     2521 gcggggatgg cgtggccaca ctgtatgtga aggtgctgct gcagatcgtg aaggacaaga
     2581 acgacgtgta cgtggacgtg tccaggcgga gcggcagctc tcatatgtct cccggaagcg
     2641 gaggccagaa gggaaaagag ggcaagaagg ccaacggcag aagcgagtcc ggcaaagaag
     2701 aggaagagga agaggacgcc gaggaaagcg acgacgacga gtacatgcgg aagtcccaga
     2761 gcgccatggg aggcggcgga tacggctata gatctggcgc cgagacagac agcgactccg
     2821 acagcgatag cgattccgat ggcggcagag ccgccgtgaa cagatacgcc tacgtggaac
     2881 tgcacggcgg agcccagaac aaggccgcca atgaagccgc caacgacgcc gctacatggg
     2941 gcgctgctaa agagcctctg agcctgctgc aagtgcgcga ggacctggac ggcgacagca
     3001 tgggcaacta ctacaagtcc agaaacggct tcttcaagtc catcttcaag cgggtgttca
     3061 agaagaaggg cgacagcgac gaggatgccg gcggaggcga cgatgaggac tccgatgaag
     3121 aacctcaggg gggcaagaaa aagcggcggt ggcggttccc ttggaagcgg cggagaggaa
     3181 aaggcagcca gctgcagggg ggagatgaca acgatgacga gggcgagtcc gaggacgaga
     3241 gcagaagcac cagaagaagg cggggaggca gacggggcct gttcggcaga tccaacagaa
     3301 agggcagagg caagggcagg gacgagtctg atgacgggga tgacggcgag gaaggggagg
     3361 attccgacga cgaagaagcc agcggcggct caggacagag aggcgctaag aagggcaagc
     3421 ggaacggcaa gggaagcggc accaaaggcg gcagattcga agagacaaag tccaagatgg
     3481 gctccctgtt cgccaaagtg aagcggaaga tcctgcccgt gaagcagaag ctgcacatcg
     3541 aggccttttt caacagcatc atcgtgaaaa gctgccggaa cgccctgaag tgggagggca
     3601 acatgttcag aaagcagagc ctggtggaaa tgaccctgaa ggtgccagtg aagctgaagt
     3661 acatcaaggg ggagcccctg cacttcttcc gcagcggcta cgaagtgatc ctgacctgcc
     3721 gcaactgtga cgaggtgctg ttcaacagct gcgtgcaggt gtactgcgcc aagcgggcca
     3781 gcaaagagga acgggccgtg gctcatcatg gcggacatgc tgatggcggc gcactgggaa
     3841 gtggcgctac agccgctgca atcacagccg cctctactgc cgcagccaca gctgctgctt
     3901 ctcccgccag cccttacctg tactctgcca gcccaagcga cgtgtccccc ctggccatga
     3961 tccactacaa cggccagtac ttccccggca gcgtgacccc tgaggccgac tacttttctt
     4021 ctggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct
     4081 ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga
     4141 aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc
     4201 agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga
     4261 taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag
     4321 ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca
     4381 ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg
     4441 agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag
     4501 caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc
     4561 aggttttatc caaagggttg aattccggat cactgcatca tattctggat gcacagaaaa
     4621 tggtgtggaa tcatcgttaa gtctagacac gtgattaatt aaggatcccc cgacctcgac
     4681 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     4741 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     4801 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     4861 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     4921 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     4981 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     5041 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     5101 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     5161 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     5221 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     5281 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     5341 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     5401 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     5461 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     5521 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     5581 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     5641 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     5701 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     5761 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     5821 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     5881 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     5941 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     6001 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     6061 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     6121 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     6181 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     6241 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     6301 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     6361 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     6421 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6481 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     6541 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     6601 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     6661 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     6721 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     6781 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     6841 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     6901 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     6961 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     7021 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7081 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7141 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7201 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7261 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7321 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7381 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     7441 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7501 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7561 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7621 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7681 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     7741 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7801 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7861 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7921 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7981 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8041 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8101 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8161 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8221 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8281 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8341 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8401 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8461 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8521 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8581 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8641 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8701 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8761 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8821 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8881 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8941 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     9001 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     9061 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     9121 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     9181 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     9241 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     9301 aggtatgga
//
