LOCUS       sequence-120660-        7575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax PVX_116775 ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: PVX_116775-bio
FEATURES             Location/Qualifiers
     source          1..7575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2955..3010
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3009..3028)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5246..5264)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5332..5436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5437..5505,5506..6297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5655..5674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6468..7056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6957..6976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7210..7227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7344..7365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7380..7385,7386..7403,7404..7410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7418..7434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7423..7445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7442..7458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7442..7458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgaag aagtgaagca caagctgggc ctgagcccca
     1381 tcctgttcac caacgcccag ggcatcggca ccaacgactg gttcaacgat tggaagaacg
     1441 acatgctggg cgactgggag aaggacttca tcagcggcat cgagaagaac tggcccagag
     1501 gctggaagaa ggatctgctg agcagcttcc agaacagctg gctgggcggc atcaacagcg
     1561 actccatgct gcgggacagc atcaacatca tcctggccca cgcccccaag aagaaccggc
     1621 agaagatcaa cgagtacatc aacgagctgc tgagcggccg gtacaacgaa gtgaacccca
     1681 agagcctgct gcaccggatc agcaatatca tcaagaacgg caaggccaag tccctgtgcg
     1741 acgacaacaa ggccttcatc atcatcggaa tcccccacct gatcttcggc ctggacctgt
     1801 tcaacgacct gaagctggaa accatcaact ttctgatccg gaatatcgcc aagcacctga
     1861 acggctgcca gctgcaggtg caggccagcg acgtgtccaa cattgccaat ggctgccaga
     1921 gcgacatgga caagcagatc atcttcgacg acgagaagcc cgaccaagtg atccacgccg
     1981 acggcctgag actgagcccc tccaacaagt tcttcagctc cctgctgcag tgcagcgtgg
     2041 acaatctggg cagcggcgag aaaaccatga tctgcgtgca ggaacagctg gccaagcgga
     2101 acctgaagct gagcgatacc tgcagcacct gtttcaaaga aagcgtggac tgcggcaaga
     2161 gcaactgctg gctgccttgc ctgttcggca acccctgcgc cgacagatgc taccagtgtg
     2221 ccaccgacag ctgcaacaaa gaactgatca gatgcagcgg cctggaaaac ctgcctggcg
     2281 cttgttctgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     2341 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     2401 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     2461 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     2521 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     2581 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     2641 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     2701 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     2761 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     2821 agatccaggt tttatccaaa gggttgaatt ccggatcact gcatcatatt ctggatgcac
     2881 agaaaatggt gtggaatcat cgttaagtct agacacgtga ttaattaagg atcccccgac
     2941 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3001 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     3061 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     3121 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     3181 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     3241 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     3301 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     3361 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     3421 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     3481 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     3541 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     3601 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     3661 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     3721 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     3781 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     3841 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     3901 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     3961 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     4021 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     4081 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     4141 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     4201 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     4261 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     4321 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     4381 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     4441 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     4501 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     4561 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     4621 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     4681 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4741 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     4801 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     4861 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     4921 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     4981 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5041 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     5101 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5161 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     5221 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5281 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5341 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5401 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5461 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5521 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5581 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5641 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5701 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5761 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5821 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5881 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5941 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     6001 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6061 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6121 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6181 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6241 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6301 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6601 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7081 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7141 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7201 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7261 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7321 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7381 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7441 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     7501 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     7561 actggtaggt atgga
//
