LOCUS       sequence-120662-        8490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax RBP2-like (PVX_101590) ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 8490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: RBP2-like-bio
FEATURES             Location/Qualifiers
     source          1..8490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3870..3925
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3924..3943)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6161..6179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6247..6351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6352..6420,6421..7212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6570..6589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7383..7971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7872..7891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8125..8142
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8259..8280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8295..8300,8301..8318,8319..8325)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8333..8349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8338..8360
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8357..8373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8357..8373
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaagg acgtgaacag aaacaagccc ggcagactga
     1381 agtaccaccg gaagctgctg cccgcctacg tgaacctgat gagccccgac aaggacggct
     1441 acaagggcgc cgagaaggac gacaaggcca acaaccacaa cgacaacaac aatgggaaca
     1501 acaacgataa caacaacaac gccgacaaca acgacgagaa ctccaacaat gccgagaacc
     1561 tgcggaccag caacctgcag aatagcgcct ctgtgggcca cctgaacaac cacgaggaca
     1621 ccaccaagcc cagccactac agctacctga agaagtccaa catctacgcc cccgacgcca
     1681 agaacaacaa gatggaagat gacaacaaga aactgcacac cgtgtccaac agcttcatcc
     1741 agagcaccgt gggcaacaac atcaacaata tcatcaaccg gctgcggcac gtgtccgtga
     1801 tcgactatga taacaatatc gtgtgcgtga tcagccccta ccaccccaaa gagtactacc
     1861 tgaccgagat gaagaatatc gccctgcagt acggcgagct gggcaacttc atcatcagcg
     1921 actacaaccc ccagatccag cacatcagcc agatcgtgcg gaagaacgtg gacatctgca
     1981 gcaaccacct gaaagaactg tacaccaccg tgtaccggct ggaaaacccc gagcggcaca
     2041 agttccagcg ggcccactac tccaacgagc ggaacgagta ccggaagaga ctggccagct
     2101 tccacgtgtg cctgcggctg atccacagaa agaactaccg ggccatcacc tacgccgagc
     2161 tgatcatcct gaacaccctg gacagcatca gctgcaactg ggacaacaac tgcgccacaa
     2221 atatctacgc caacatcctg aaaacctaca tgagcgagat cagcaacttc gagaagaaga
     2281 aaaagaacga cgccatcaac aagattaaca acatctacaa gagcgccgtg gatgccatca
     2341 ccaaaatcaa gagcgagctg acccccagcg tgaccagcga caccgccgat ttcgtgctgg
     2401 acgagatcaa gtacatcatc gacaagatca acatccacct ggaaaagatt aagtacggca
     2461 gcgagtacgt gaagtatatc aacagcgaga agatccccca gcagtacaag aaggatgacc
     2521 tgaagtccca ctacgtggtg ctcgtgacac actacagcgc ctttctgttc tccaccaagc
     2581 acatcaacgt gctggaagat gtgttcaaga acaaagagcg gatcctgtac gacgtgttcg
     2641 gcaagatcga gagcgccctg cggaacaagc tgaccaccct gatcgactcc agcttcagcg
     2701 tgtccacctg taacagcgcc atcagcatct gcgaagagat caaaagcgcc gagcccctgc
     2761 tggcctctag cgccaagcct atcgagagac accggatcta cagcaacgcc gagatcaaag
     2821 aggccaagaa gaagtacgac gaaaagatcg tgaaactgga acaggccatc cggaaggccg
     2881 agggcttcat caaccccctg aaggacatca tccagttcaa cattgcccag aaagaagcca
     2941 tggaaaaccg gatcaatcag atccagaagg gcctgaacgc tctggaaacc gaccggaaga
     3001 tcctggaaat catcgatgcc attaacaacc agaagtccca gatctccaag aatagcaaga
     3061 agatcaagga cagcagccgg accgccaatg ccctgaaaac acaggtgcag accctgaaga
     3121 aagaaatcga cgacgatgtg aaactgctgg tgcagctgat cgagaaagag aagatcgccg
     3181 ccttcctgcg cgaggaaatc aaaggcgcgc cgtcgacctc catcacggcc tataagagtg
     3241 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     3301 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     3361 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     3421 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     3481 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     3541 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     3601 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     3661 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     3721 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccgga tcactgcatc
     3781 atattctgga tgcacagaaa atggtgtgga atcatcgtta agtctagaca cgtgattaat
     3841 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     3901 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     3961 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     4021 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     4081 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     4141 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     4201 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     4261 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     4321 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     4381 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     4441 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     4501 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     4561 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     4621 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     4681 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     4741 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     4801 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     4861 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     4921 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     4981 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     5041 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     5101 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     5161 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     5221 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     5281 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     5341 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     5401 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     5461 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     5521 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     5581 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5641 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     5701 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5761 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     5821 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     5881 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     5941 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     6001 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6061 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     6121 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     6181 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6241 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6301 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6361 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6421 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6481 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6541 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6601 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6661 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6721 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6781 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6841 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6901 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6961 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7021 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7081 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7141 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7201 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7261 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7321 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7381 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7441 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7501 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7561 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7621 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7681 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7741 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7801 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7861 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7921 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7981 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8041 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8101 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8161 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8221 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8281 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8341 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     8401 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     8461 tgctgcaggc gcagaactgg taggtatgga
//
